长链非编码RNA MALAT1对人肺成纤维细胞向肌成纤维细胞转化的影响  

Effects of Long Non⁃Coding RNA MALAT1 on the Transformation of Human Lung Fibroblasts Into Myofibroblasts

作　　者：常清 陈飞[1] 张铁栓[2] 乔华[1] 万军营 CHANG Qing;CHEN Fei;ZHANG Tie-shuan;QIAO Hua;WAN Jun-ying(Department of The First Pulmonary and Critical Care Medicine,Nanyang First People’s Hospital,He’nan Nanyang 473004,China;Department of Respiratory Medicine,The Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China;Department of Pulmonary and Critical Care Medicine,Zhengzhou Zhongmu County People’s Hospital,Zhengzhou 451450,China)

机构地区：[1]南阳市第一人民医院呼吸与危重症医学一科,河南南阳473004 [2]郑州大学第二附属医院呼吸内科,郑州450014 [3]郑州市中牟县人民医院呼吸与危重症医学科,郑州451450

出　　处：《循证医学》2023年第2期103-108,共6页The Journal of Evidence-Based Medicine

摘　　要：目的探索长链非编码RNA(long non-coding RNA,lncRNA)肺腺癌转移相关转录本1(metastasisassociated lung adenocarcinoma transcript 1,MALAT1)对人肺成纤维细胞向肌成纤维细胞转化的影响。方法培养人肺成纤维细胞(human fetal lung fibroblast 1,HFL-1),按照有无转化生长因子-β1(transforming growth factor-β1,TGF-β1)作用分为观察组和对照组,检测两组细胞中lncRNA MALAT1表达水平。在此基础上设置四个组别:空白组、TGF-β1组、转染对照组和siMALAT1组。空白组细胞不接受任何处理;TGF-β1组采用5 ug/L的TGF-β1处理;转染对照组在TGF-β1组的基础上应用siMALAT1阴性对照(negative control,NC)处理;siMALAT1组在TGF-β1组的基础上应用siMALAT1。比较其lncRNA MALAT1、Ⅰ型胶原(collagen 1,COL1)和α-平滑肌肌动蛋白(α-smooth muscle activation protein,α-SMA)的mRNA表达水平,COL1和α-SMA的蛋白表达水平和细胞增殖情况。结果观察组lncRNA MALAT1相对表达量较对照组高(P<0.05)。四组细胞lncRNA MALAT1、α-SMA和COL1的mRNA及蛋白相对表达量有统计学差异(P<0.05);其中,转染对照组和TGF-β1组以上RNA和蛋白相对表达量均高于siMALAT1组和空白组(P<0.05),而TGF-β1组与转染对照组之间上述RNA和蛋白相对表达量则无差异(P>0.05)。四组细胞增殖活力的差异具有统计学意义(P<0.05);转染对照组和TGF-β1组细胞增殖活力较siMALAT1组和空白组高(P<0.05),而TGF-β1组与转染对照组之间则无差异(P>0.05)。结论lncRNA MALAT1可以促进人肺成纤维细胞向肌成纤维细胞的转化,影响肺纤维化进程中COL1、α-SMA在肺组织中的沉积,靶向lncRNA MALAT1将有助于对肺纤维化过程的深入研究。Objective To explore the effect of long noncoding RNA(lncRNA)metastasis⁃associated lung adenocarcinoma transcript 1(MALAT1)on the transformation of human lung fibroblasts into myofibroblasts.Methods The cultured human lung fibroblasts 1(HFL⁃1)were divided into an observation group and a control group according to whether they have the effect of transforming growth factor⁃β1(TGF⁃β1).Detected the expression levels of lncRNA MALAT1 in cells of the two groups.On this basis,four clusters were established:blank group,TGF⁃β1 group,transfection control group,and siMALAT1 group.Cells in the blank group did not receive any treatment;the TGF⁃β1 group was treated with TGF⁃β1 at 5μg/L;the transfection control group was treated with siMALAT1 negative control(NC)based on the TGF⁃β1 group;siMALAT1 group was treated with siMALAT1 based on the TGF⁃β1 group.The lncRNA MALAT1,mRNA expression levels of collagen 1(COL1)andα⁃smooth muscle activation protein(α⁃SMA),protein expression levels of COL1 andα⁃SMA,and cell proliferation were compared among them.Results Compared with the control group,lncRNA MALAT1 relative expression in the observation group was higher(P<0.05).There were statistically significant differences in the four groups on lncRNA MALAT1,mRNA expression levels of COL1 andα⁃SMA,and protein expression levels of COL1 andα⁃SMA(P<0.05).The relative expression of RNA and protein above in the transfection control group and TGF⁃β1 group was higher than that in the siMALAT1 group and blank group(P<0.05),but there were no significant between the TGF⁃β1 group and the transfection control group(P>0.05).The four groups showed significant differences in cell viability(P<0.05).The proliferation activity of the transfection control group and TGF⁃β1 group was higher than that of the siMALAT1 group and blank group(P<0.05),however,there were no significant between the TGF⁃β1 group and the transfection control group(P>0.05).Conclusions LncRNA MALAT1 can promote the transformation of human

关 键 词：长链非编码RNA 肺腺癌转移相关转录本1 特发性肺纤维化 

分 类 号：R563[医药卫生—呼吸系统]