离子色谱法测定布洛芬原料药中2-氯丙酸根及2,2-二氯丙酸根的含量  

Content determination of 2-chloropropionate and 2,2-dichloropropionate in ibuprofen by ion chromatography

作　　者：邢雪敏 刘葵葵 李欣 刘梦瑶 张琳 XING Xue-min;LIU Kui-kui;LI Xin;LIU Meng-yao;ZHANG Lin(Shandong Academy of Pharmaceutical Sciences,Shandong Key Laboratory of Chemical Drug,Shandong Freda Pharmaceutical Group Co.,Ltd.,Shandong Key Laboratory of Mucosa and Transdermal Drug Delivery Technology,Jinan 250101)

机构地区：[1]山东省药学科学院山东省化学药物重点实验室山东福瑞达医药集团有限公司山东省黏膜与皮肤给药技术重点实验室,济南250101

出　　处：《中南药学》2023年第9期2463-2465,共3页Central South Pharmacy

基　　金：山东省重点研发计划(No.2021ZDSYS07)。

摘　　要：目的建立离子色谱法测定布洛芬原料药中2-氯丙酸根及2,2-二氯丙酸根的含量。方法采用Thermo Dionex IonPac AS18(4 mm×250 mm)色谱柱,以20 mmol·L^(-1)氢氧化钾溶液为淋洗液,流速为1.0 mL·min^(-1),进样体积为25μL;采用抑制电导检测法,抑制电流为52 mA。结果2-氯丙酸根离子在0.00486~4.86μg·mL^(-1)内,2,2-二氯丙酸根离子在0.0400~4.00μg·mL^(-1)内与峰面积呈良好的线性关系;2-氯丙酸根离子、2,2-二氯丙酸根离子平均回收率分别为104.4%、101.6%,RSD分别为0.40%、1.8%。结论本方法简便、准确、快速,适用于布洛芬原料药中2-氯丙酸根及2,2-二氯丙酸根的含量测定。Objective To determine the content of 2-chloropropionate and 2,2-dichloropropionate in ibuprofen by ion chromatography.Methods Thermo Dionex IonPac AS18(4 mm×250 mm)column was used with the mobile phase consisting of 20 mmol·L-1 KOH at 1.0 mL·min^(-1) and the injection volume was 25μL.Suppressed conductivity detector was used with 52 mA suppression current.Results A good linearity was shown when the sample of 2-chloropropionate was within the range of 0.00486~4.86μg·mL^(-1) and 2,2-dichloropropionate within the range of 0.0400~4.00μg·mL^(-1).The average recovery of 2-chloropropionate and 2,2-dichloropropionate was 104.4%and 101.6%with RSD of 0.40%and 1.8%,respectively.Conclusion The method is convenient,accurate and fast,which can be used for the content determination of 2-chloropropionate and 2,2-dichloropropionate in ibuprofen.

关 键 词：离子色谱法 布洛芬 2-氯丙酸根 2 2-二氯丙酸根 含量测定 

分 类 号：R927[医药卫生—药学]