小麦HP基因家族鉴定和分析  

Identification and analysis of HP gene family in wheat

作　　者：娄渊根 李闯[2] 李晶晶 邢国珍[2] 路亚南 郑文明[2] LOU Yuangen;LI Chuang;LI Jingjing;XING Guozhen;LU Yanan;ZHENG Wenming(Henan Vocational College of Nursing,Anyang 455000,Henan,China;College of Life Sciences/State Key Laboratory of Wheat and Maize Crop Science,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南护理职业学院,河南安阳455000 [2]河南农业大学生命科学学院/小麦玉米作物学国家重点实验室,河南郑州450002

出　　处：《浙江农业学报》2023年第9期2023-2032,共10页Acta Agriculturae Zhejiangensis

基　　金：小麦玉米作物学国家重点实验室课题“小麦抗病与养分高效种质资源发掘利用”。

摘　　要：组氨酸磷酸转运蛋白HP(histidine phosphotransfer proteins)在植物的生长发育调控及逆境胁迫应答中发挥重要作用。为理解HP基因在小麦基因组中的进化特征和功能,研究通过生物信息学分析鉴定普通小麦的HP基因家族成员,对其理化性质、进化特征、基因结构、顺式作用元件以及在逆境胁迫条件下的表达模式进行了分析。结果表明,在小麦基因组鉴定到31个HP基因,编码蛋白质序列包括116~200个氨基酸。通过和其他植物的HP蛋白比较以及对蛋白结构、基因结构和基序的分析,显示小麦HP家族基因序列具有保守性。顺式作用元件预测表明,HP基因具有光、植物激素、干旱、低温等非生物胁迫响应相关的启动子调控元件。热图分析表明,HP基因在应答非生物胁迫中表达模式存在多样性;qRT-PCR分析表明,TaHP5-6B基因在低磷胁迫下受到强烈的诱导表达。综上所述,小麦全基因组鉴定的HP家族基因是非生物胁迫响应的重要基因资源。HP(histidine phosphotransfer proteins)plays an important role in regulating growth and development and responding to stress in plants.In order to understand the evolution and function of HP genes in wheat genome,this study identified the HP gene family for Triticum aestivum by the bioinformatics method,and analyzed the physicochemical properties,evolutionary characteristics,gene structure,cis-acting elements,and expression patterns under stress.The results showed that 31 HP genes were identified.The length of amid acids of HP proteins ranged from 116 to 200.Comparison with HP proteins of other plants and analysis of protein structure,gene structure and motif showed that sequences of HP gene family were conserved in wheat.Analysis of cis-acting elements revealed that the promoter regions of HP genes contained regulatory elements involved in abiotic stress,such as light,phytohormone,drought and low temperature.Heatmap analysis showed that HP genes significantly differed in the expression levels under abiotic stress.qRT-PCR analysis indicated that TaHP5-6B was strongly induced under low phosphorus stress.In conclusion,HP family genes identified at the genome-wide level are important gene resources associated with abiotic stress response in wheat.

关 键 词：小麦 组氨酸磷酸转运蛋白 保守序列 HP基因家族 低磷胁迫 

分 类 号：S512.1[农业科学—作物学]