玫烟色棒束孢转录组测序及潜在致病相关基因分析  

Transcriptome sequencing and analysis of potential pathogenicity-related genes in Isaria fumosorosea

作　　者：谢梅琼 王龙江 何余容 吕利华[4] XIE Meiqiong;WANG Longjiang;HE Yurong;LYU Lihua(College of Life Sciences and Resources and Environment,Yichun University,Yichun 336000,Jiangxi,China;College of Chemistry and Bioengineering,Yichun University,Yichun 336000,Jiangxi,China;College of Plant Protection,South China Agricultural University,Guangzhou 510642,China;Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Guangzhou 510642,China)

机构地区：[1]宜春学院生命科学与资源环境学院,江西宜春336000 [2]宜春学院化学与生物工程学院,江西宜春336000 [3]华南农业大学植物保护学院,广东广州510642 [4]广东省农业科学院植物保护研究所,广东广州510642

出　　处：《浙江农业学报》2023年第9期2169-2180,共12页Acta Agriculturae Zhejiangensis

基　　金：江西省重点研发计划(20202BBFL63009);江西省教育厅项目(GJJ201613,GJJ201620)。

摘　　要：本研究利用Illumina HiSeq高通量无参转录组测序对玫烟色棒束孢IFCF01菌株进行测序及分析,探讨玫烟色棒束孢转录组基因的表达特征,为该菌基因功能研究提供基础数据。转录组测序共获得540896444条序列片段。组装后共获得36466条基因序列集合,总长度为34551468 bp,平均长度947 bp。功能注释结果显示,共有22741个基因在Nr、GO、COG、KEGG等6大数据库被注释,占总数的62.36%。在Nr数据库中共有21737条基因得到注释,其中注释到球孢白僵菌的数量最多,为44.3%。共有10888个基因注释到COG数据库的25个功能类别,匹配上4060个基因序列。GO注释结果显示,共有9617个基因得到注释,分布在生物过程、分子功能、细胞组分3大类功能模块的49个亚类。共有14364个基因在KEGG数据库得到注释,涉及108个KEGG代谢途径。利用病原菌和寄主互作数据库,共鉴定到86条玫烟色棒束孢潜在致病相关的基因。对基因序列进行简单重复序列(SSR)位点检测共获得8572个SSR位点,发生频率为18.18%。本研究结果可为玫烟色棒束孢基因资源挖掘、功能基因研究及SSR分子标记奠定基础。In order to explore the expression patterns of transcriptome genes of Isaria fumosorosea and to provide data for the functional study of I.fumosorosea,the transcriptom of I.fumosorosea IFCF01 was sequenced and analyzed using high-throughput non-parametric sequencing technology.A total of 540896444 clean reads were obtained by transcriptome sequencing.A total of 36466 unigenes with the total length of 34551468 bp and an average length of 947 bp were obtained after assembly.The functional annotation showed that a total of 22741 unigenes were annotated in 6 major databases,such as Nr,GO,COG and KEGG,accounting for 62.36%of the total unigenes.A total of 21737 unigenes were annotated in the Nr database,with the highest number of anotation to Beauveria bassiana.10888 unigenes were annotated to 25 functional categories in COG database,and the number of genes involved in general function predicted only was the largest,matching on 4060 unigenes.The GO annotation shoewed that a total of 9617 unigenes were annotated,distributed in 49 subcategories of three functional modules:biological process function,molecular function and cell composition.A total of 14364 unigenes were annotated in the KEGG database,involving 108 KEGG metabolic pathways.With the help of pathogen-host interaction gene database,a total of 86 unigenes associated with the potential pathogenicity of I.fumosorosea were identified.A total of 8572 simple sequence repeats(SSR)loci were detected in transcriptome data of I.fumosorosea,with a frequency of 18.18%.The results of this study could lay the foundation for gene resources mining,functional gene research and SSR molecular markers for I.fumosorosea.

关 键 词：玫烟色棒束孢 转录组测序 基因 SSR分子标记 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]