东莨菪碱调节SIN3A表达对水杨酸诱导耳鸣大鼠mGluR5/Homer1信号通路的影响  

Effect of scopolamine on the mGluR5/Homer1 signaling pathway in salicylic acid-induced tinnitus rats by regulating SIN3A expression

作　　者：颜微微 邓可斌[2] YAN Weiwei;DENG Kebin(First Clinical College,Hubei University of Chinese Medicine,Hubei,Wuhan 430000,China;不详)

机构地区：[1]湖北中医药大学第一临床学院,武汉市430000 [2]湖北省中医院耳鼻喉科

出　　处：《河北医药》2023年第19期2933-2936,共4页Hebei Medical Journal

基　　金：湖北省教育厅科研计划项目(编号:B2022122)。

摘　　要：目的探究东莨菪碱(SPL)调节SIN3A表达对水杨酸诱导的耳鸣大鼠mGluR5/Homer1信号通路的影响。方法将50只大鼠分为对照组、模型组、阳性组、SPL低、高剂量组,每组10只。分别对5组大鼠进行惊跳反射前脉冲抑制(PPI)检测和听性脑干反应(ABR)检测;RT-qPCR检测大鼠听皮层组织中SIN3A的mRNA水平;Western blot检测大鼠听皮层组织中SIN3A、mGluR5、Homer1蛋白的表达。结果与对照组相比,模型组大鼠的前脉冲抑制率、ABR阈值、mGluR5和Homer1的蛋白表达均显著升高(P<0.05),SIN3A mRNA水平和蛋白表达降低(P<0.05);与模型组相比,阳性组和SPL处理组大鼠的前脉冲抑制率、ABR阈值、mGluR5和Homer1的蛋白表达均显著降低(P<0.05),SIN3A mRNA水平和蛋白表达升高(P<0.05)。结论SPL可以通过促进SIN3A的表达,来抑制水杨酸诱导的耳鸣大鼠mGluR5/Homer1信号通路。Objective To investigate the effect of scopolamine(SPL)on the mGluR5/Homer1 signal pathway in salicylic acid-induced tinnitus rats.Methods A total of 50 rats were randomly divided into control group,model group,positive group,low-dose SPL group and high-dose SPL group,with 10 in each group.Pre-pulse inhibition(PPI)test and auditory brainstem response(ABR)test were performed in all rats.The mRNA level of SIN3A in rat auditory cortex was detected by reverse transcription real-time polymerase chain reaction(RT-qPCR),and protein expressions of SIN3A,mGluR5 and Homer1 in the auditory cortex of rats were detected by Western blot.Results Compared with the control group,rats in the model group presented significantly higher PPI rate,ABR threshold and protein expressions of mGluR5 and Homer1,and significantly lower mRNA and protein levels of SIN3A(all P<0.05).Compared with the model group,rats in the positive group,low-dose SPL group and high-dose SPL group presented significantly lower PPI rate,ABR threshold and protein expressions of mGluR5 and Homer1,and significantly higher mRNA and protein levels of SIN3A(all P<0.05).Conclusion SPL can inhibit the mGluR5/Homer1 signal pathway in salicylic acid-induced tinnitus rats by upregulating SIN3A.

关 键 词：东莨菪碱 SIN3A mGluR5/Homer1信号通路 耳鸣大鼠 

分 类 号：R764.45[医药卫生—耳鼻咽喉科]