基于空间位移拉曼光谱的皮肤含水量无创检测  

Noninvasive Detection of Skin Moisture Content Based on Spatially Offset Raman Spectroscopy

作　　者：袁榕穗 吴植强 聂荣志 钟文婷 张克勤[4] YUAN Rongsui;WU Zhiqiang;NIE Rongzhi;ZHONG Wenting;ZHANG Keqin(Guangxi Food and Drug Evaluation&Inspection Center,Nanning 530000,China;Guangxi Medical Device Testing Center,Nanning 530000,China;Department of Public Foundation Education,Hezhou University,Hezhou 542899,China;College of Textile and Clothing Engineering,Soochow University,Suzhou 215021,China)

机构地区：[1]广西食品药品审评查验中心,南宁530000 [2]广西医疗器械检测中心,南宁530000 [3]贺州学院公共基础教学部,贺州542899 [4]苏州大学纺织与服装工程学院,苏州215021

出　　处：《光子学报》2023年第9期121-129,共9页Acta Photonica Sinica

基　　金：国家自然科学基金(No.51873134)。

摘　　要：基于空间位移拉曼光谱原理,搭建了一套可以实现微小空间位移的装置,确定了最优的空间位移距离为300μm,用于穿透皮肤表层,减少皮肤表面水份、油脂等污染物的干扰,最终采集到皮肤内层的水份拉曼信号。该装置采用拉曼波数在3390 cm^(−1)(水拉曼峰)和2935 cm^(−1)(蛋白峰)处的强度之比确定组织中的水-蛋白比例,采用便携式光谱仪使设备便于移动,探头和光谱仪以光纤相连使探头可灵活操作,设计满足临床使用要求。实验中检测了7个人、每人10个共70个不同位置的皮肤光谱,将光谱进行去背景算法处理,然后得到精确的拉曼峰强度。以电学法皮肤水份测试仪测试结果作为对比,以组内相关系数作为一致性分析指标,由单个测量组内相关系数为0.889可以看出,基于空间位移拉曼光谱法的人体活体皮肤含水量检测具有较高可行性。In this study,the feasibility of detecting skin water content based on spatial displacement Raman spectroscopy and portable fiber optic spectrometer was explored.A device was constructed that can achieve an optimal spatial displacement distance of 300μm.It is selected to detect signals noninvasively under the surface of the skin and reduce the interference of pollutants such as water and oil on the surface of the skin.Instead of using the traditional spatial shift Raman spectroscopy device to achieve the relative shift of excitation point and collection point by mechanical offset or light blocking,a micro-shiftable light source was set up to adjust the spatial shift by using the conjugate relationship between the light source and the excitation point,and at the same time,a microscopic imaging device was introduced to control the adjustment,which finally realized the continuous adjustable spatial shift of less than 1000μm.The ratio of the intensity of Raman shift at 3390 cm^(−1)(water Raman peak)and 2935 cm^(−1)(protein Raman peak)is employed to characterize the skin moisture content,a portable spectrometer is employed to make the device moveable,the probe and the spectrometer are connected by optical fiber to make the probe flexible.Therefore,the design meets the clinical requirements.In total,70 spectra from different locations were collected(7 people,10 spectra each)in the experiment,and the spectra were processed by a de-backgrounding algorithm,and then the exact Raman peak intensity was obtained.The intra-group correlation coefficient was used as an index for consistency analysis.The intra-group correlation coefficient of 0.889 for a single measurement showed that the results of the two methods were in good agreement.In this study,four experiments with different spatial displacement amounts were set up,and the spatial displacement distances were 200μm,300μm,500μm and 1000μm.The skin spectra from the same person at the same location measured under different spatial displacement amounts can be seen to

关 键 词：空间位移拉曼光谱 皮肤含水量 无创检测 一致性分析 组内相关系数 

分 类 号：O433.4[机械工程—光学工程]