Methanolic pomegranate dried peel extract improves cryopreserved semen quality and antioxidant capacity of rams  

作　　者：Amal M.Aboelmaaty Mohamed S.Kotp Aya M.Fadl Elshymaa A.Abdelnaby Islam E.El-Seadawy Hossam R.El-Sherbiny 

机构地区：[1]Animal Reproduction and Artificial Insemination Department,Veterinary Research Institute,National Research Centre,Cairo,Egypt [2]Theriogenology Department,Faculty of Veterinary Medicine,Cairo University,Cairo,Egypt

出　　处：《Asian pacific Journal of Reproduction》2023年第5期229-238,共10页亚太生殖杂志（英文版）

摘　　要：Objective:To select the appropriate concentrations of methanolic pomegranate extract supplemented in rams’semen extender for obtaining the best-cryopreserved semen quality.Methods:Tris-based semen extender was supplemented with 0.0,0.40,0.48,and 0.56 mg/mL pomegranate peel methanolic extract to extend semen collected from five native rams twice weekly for two months(n=80).Pooled(n=16)post-thaw semen characteristics were determined.Thawed seminal plasma of all supplemented and control groups were used to measure malondialdehyde(MDA),nitric oxide(NO),glutathione peroxidase(GPx),superoxide dismutase(SOD),ascorbic acid,zinc,copper,total cholesterol,low-density lipoproteins(LDL),lactate dehydrogenase(LDH),and alkaline phosphatase(ALP).Results:The supplementation of Tris-based semen extender with 0.48 mg/mL semen extender resulted in the highest post-thaw sperm total motility(P<0.001),sperm progressive motility(P<0.001),live sperm(P<0.001),sperm plasma membrane integrity(P<0.001),acrosome integrity(P<0.001),SOD(P<0.05),zinc(P<0.001),total cholesterol(P<0.001),and LDL(P<0.001)with the lowest percentage of abnormal sperm morphology(P<0.001),the lowest lipid peroxidation(MDA,P<0.01),ascorbic acid(P>0.05),and LDH(P>0.05).Conclusions:Pomegranate peel methanolic extract 0.48 mg/mL supplemented to Tris-based semen extender of rams is the best enrichment in preserving the sperm post-thaw characteristics via improving biochemical profiles and antioxidant capacity.

关 键 词：Oxidants-antioxidants Pomegranate peel ethanolic extract Post-thaw semen characteristics Tris-based semen diluents RAMS 

分 类 号：R285.5[医药卫生—中药学]