NO对不同盐碱胁迫下藜麦幼苗生长及氮代谢的影响  

Effects of nitric oxide on growth and nitrogen metabolism of quinoa seedlings under saline alkali stress

作　　者：冯垚 赵颖[1,2] 魏小红 FENG Yao;ZHAO Ying;WEI Xiaohong(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China;State Key Laboratory of Aridland Crop Science,Gansu Agricultural University,Lanzhou,730070,Gansu,China)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]省部共建干旱生境作物重点实验室甘肃农业大学,甘肃兰州730070

出　　处：《草业科学》2023年第9期2320-2329,共10页Pratacultural Science

基　　金：甘肃农业大学干旱生境作物学重点实验室开放基金课题(GSCS-2021-10);甘肃省自然科学基金(2IJR7RA808)。

摘　　要：为探究盐碱胁迫下藜麦(Chenopodiumquinoa)幼苗对外源NO的生理响应,以‘陇藜4号’为试验材料,用NO释放剂硝普纳(SNP,150μmol·L^(-1))和5种不同pH(7.00、7.62、8.98、9.18、9.75、10.53)的混合盐碱溶液(200 mmol·L^(-1))对八叶一心期的藜麦幼苗进行处理,从藜麦幼苗生长、氮代谢相关酶活性及含氮化合物3个方面研究外源NO对盐碱胁迫下藜麦幼苗生长及氮代谢的影响。结果表明:藜麦幼苗在盐碱胁迫下株高、生物量、硝酸还原酶(NR)和谷氨酰胺合成酶(GS)活性均呈下降趋势,且随着盐碱胁迫的加剧,下降幅度增大;但根长、根冠比及可溶性蛋白含量和游离氨基酸总含量均呈升高趋势,其含量随盐碱胁迫的加剧而增加;外施NO后,可促进藜麦的地上及地下部分生长,提高氮代谢相关酶活性及含氮化合物含量(除可溶性糖含量呈下降趋势外)。其中,碱性盐处理中pH 9.75的处理效果最明显,其根长、生物量、NR和GS活性分别增加了110%、340%、51.52%和83.08%。综上所述,NO对盐碱胁迫下藜麦幼苗的生长与氮代谢有明显的缓解作用,且对多组分的碱性盐的缓解效果优于中性盐。In order to explore the physiological response of quinoa seedlings to exogenous NO under saline alkali stress,Longli No.4 was used as an experimental material to treat quinoa seedlings with eight leaves at one heart stage with SNP and five mixed saline alkali solutions with different pH(200 mmol·L^(-1)).The effects of exogenous NO(150μmol·L^(-1))on the growth and nitrogen metabolism of quinoa seedlings under saline alkali stress were studied from three aspects:quinoa seedling growth,and the activity of enzymes related to nitrogen metabolism and nitrogen-containing compounds.The results showed that the plant height,biomass,and nitrate reductase(NR)and glutamine synthetase(GS)activities of quinoa seedlings showed a downward trend under saline alkali stress,and a greater decline with the intensification of saline alkali stress.However,the root length,root:shoot ratio,soluble protein content,and total free amino acid content all showed an increasing trend,and their content increased with an increase in salinity.NO could promote the growth of the aboveground and underground parts of quinoa,and increase the activity of enzymes related to nitrogen metabolism and the content of nitrogen-containing compounds(although the content of soluble sugar showed a downward trend).Among the alkaline salt treatments,treatment D(pH 9.75)had the most obvious effect,and its root length,biomass,and NR and GS activities increased by 110%,340%,and 51.52%,and 83.08%respectively.In conclusion,NO can significantly alleviate the growth and nitrogen metabolism of quinoa seedlings under saline alkali stress,and its alleviating effect on multi-component alkaline salt is better than that of neutral salt.

关 键 词：藜麦 硝普钠 混合盐碱 pH 生长指标 氮代谢酶 含氮化合物 

分 类 号：S519[农业科学—作物学]