镉胁迫对不同谷子品种种子萌发特性的影响  

Effects of cadmium stress on seed germination characteristics of 58 millet accessions

作　　者：范利荣 晁赢 阎祥慧[1] 王建新 诸葛玉平[1] 娄燕宏[1] 王会[1] 潘红 杨全刚[1] FAN Lirong;CHAO Ying;YAN Xianghui;WANG Jianxin;ZHUGE Yuping;LOU Yanhong;WANG Hui;PAN Hong;YANG Quangang(National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources/College of Resources and Environment of Shandong Agricultural University,Taian 271018,Shandong,China;Ministry of Science and Technology Innovation,Taian Hi-tech Industrial Development Zone,Taian 271000,Shandong,China;Daiyue Bureau of Agriculture and Rural Affairs,Taian 271000,Shandong,China)

机构地区：[1]土肥资源高效利用国家工程研究中心/山东农业大学资源与环境学院,山东泰安271018 [2]泰安高新技术产业开发区科技创新部,山东泰安271000 [3]岱岳农业农村局,山东泰安271000

出　　处：《草业科学》2023年第9期2330-2339,共10页Pratacultural Science

基　　金：山东省现代农业产业技术体系杂粮创新团队栽培与土壤肥料岗位专家经费项目(SDAIT-15-04);山东省自然科学基金重大基础研究项目(ZR2018ZC2363)。

摘　　要：为评价和比较不同来源谷子(Setaria italica)在萌发期的耐镉性,本研究选用58份谷子品种作为试验材料,以100 mg·L^(−1)镉溶液进行镉胁迫,用蒸馏水作为对照,采用双层滤纸法在人工智能培养箱中进行谷子种质萌发期的耐镉性鉴定;通过主成分分析明确各萌发指标在耐镉性评价中的权重,利用模糊数学中的隶属函数法和聚类分析法对58份谷子种质的耐镉性进行综合评价和耐镉等级划分。结果表明:不同谷子品种的耐镉性差异显著(P<0.05),胚根较胚芽对镉胁迫更敏感;谷子品种的相对根长、相对发芽率、相对发芽势对镉胁迫较为敏感,其变异系数分别为50.83%、57.56%、57.80%。主成分分析表明,发芽率、发芽势、相对发芽率、相对发芽势可作为谷子耐镉性评价的关键指标;通过聚类分析可将58份谷子种质分为耐镉型、中间型和镉敏感型3类,其中耐镉谷子品种占比20.69%,其相对发芽率、相对发芽势均≥60.00%。基于模糊数学中的隶属函数得分进行综合评价,筛选出综合得分值>5的8份耐镉型品种,其相对发芽率、相对发芽势均≥60.00%。To evaluate and compare the cadmium(Cd)tolerance of foxtail millet(Setariaitalica)accessions from different origins during the germination period,in this study,58 accessions of foxtail millet were employed as the subject of the experiment,100 mg·L^(−1)Cd solution was used to apply Cd stress,and distilled water was used as the control.The Cd tolerance of the millet germplasm was determined following a double-layer filter paper method in an artificial intelligence incubator.Through principal component analysis,the weight of each germination index in the evaluation of Cd resistance was elucidated,and the membership function and cluster analysis methods were used to comprehensively evaluate the Cd resistance via fuzzy mathematics.The results showed that the Cd tolerance of different millet varieties significantly differed,and the radicle was more sensitive to Cd stress than the germ.The relative root length,relative germination percentage,and relative germination potential of millet varieties were sensitive to Cd stress,and their coefficients of variation were 50.83%,57.56%,and 57.80%,respectively.Principal component analysis showed that the germination percentage,germination potential,relative germination percentage,and relative germination potential could be used as the key indexes for evaluating the Cd tolerance of millet.According to the cluster analysis,the 58 millet germplasms could be divided into three types:tolerant,moderately tolerant,and sensitive.Among them,Cd-tolerant millet varieties accounted for 20.69%,and their relative germination percentage and relative germination potential were all≥60.00%.Based on the membership function score in fuzzy mathematics,eight Cd-tolerant varieties with a comprehensive score of>5 were selected,and their relative germination percentage and relative germination potential were≥60.00%.

关 键 词：谷子 耐镉性 萌发期 隶属函数法 聚类分析 综合评价 

分 类 号：S515[农业科学—作物学]