甜高粱青贮替代玉米青贮对肉羊血液生理生化及抗氧化指标的影响  

Effect of sweet sorghum silage replacing maize silage on blood physiology,serum biochemical,and serum antioxidant indexes of sheep

作　　者：王斐[1] 何振富[1] 陈平 谢建鹏 贺春贵[1] WANG Fei;HE Zhenfu;CHEN Ping;XIE Jianpeng;HE Chungui(Animal Husbandry-Pasture and Green Agriculture Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃省农业科学院畜草与绿色农业研究所,甘肃兰州730070

出　　处：《草业科学》2023年第9期2363-2372,共10页Pratacultural Science

基　　金：甘肃省现代农业科技支撑体系区域创新中心重点科技项目(2020GAAS05);甘肃省农业科学院农业科技创新专项(2021GAAS45);嘉峪关市科技计划项目(21-24);甘肃省青年科技人才托举工程项目(2020-05)。

摘　　要：以50只健康小尾寒羊为研究对象,随机分为5组,每组10头,分别以0(对照组)、25%、50%、75%、100%(以干物质计)甜高粱(Sorghumbicolor)青贮替代玉米(Zeamays)青贮,饲喂90 d后测定其生长性能、血液生理生化指标及血清抗氧化指标,旨在探明甜高粱青贮以不同比例替代玉米青贮对肉羊健康水平的影响。结果显示:1)以甜高粱替代不同比例玉米青贮对肉羊平均日增重和日均采食量无显著影响(P>0.05)。2)不同处理对各组肉羊血液生理指标无显著影响;经90 d试验期后各组的中性粒细胞比率和中间细胞比率、25%组的白细胞总数、对照组和25%~75%组的血小板总数及75%组的血小板比容均较第1天显著降低(P<0.05)。对照组、25%组和75%组的淋巴细胞比率及25%组和50%组的红细胞总数均较第1天显著升高。3)各组血清谷草转氨酶和碱性磷酸酶活性均高于参考范围值;对照组的谷草转氨酶活性显著高于其他组且其他组间差异不显著;对照组的碱性磷酸酶活性显著高于50%组和100%组;75%组的白蛋白显著低于25%组,但肌酐含量显著高于25%组;其他指标各组间均无显著差异。4)50%组的过氧化氢酶活性、100%组的超氧化物歧化酶活性和75%组的总抗氧化能力均显著高于对照组;各组的谷胱甘肽过氧化物酶活性和丙二醛水平无显著差异。综上所述,以甜高粱青贮替代玉米青贮能够在一定程度上改善血清生化指标,有效保护其肝脏和肾脏,同时可提高肉羊机体抗氧化能力。This study investigated the effects of substituting sweet sorghum silage with maize silage in different proportions on sheep health.Fifty healthy sheep were randomly divided into five groups with 10 sheep in each group.Maize silage was replaced with 0(control),25%,50%,75%,and 100%sweet sorghum silage.Blood physiology and serum biochemical and antioxidant indices of the sheep were determined after feeding for 90 d.The results showed that:1)substituting sweet sorghum silage with maize silage had no significant effect on daily weight gain and average daily feed intake in sheep,and 2)different treatments had no significant negative effect on the blood physiological indexes of sheep.After 90 d of the experiment,neutrophil ratio(NEUT%)and intermediate cell ratio(MID%)in all groups,white blood cell(WBC)in the 25%group,platelet(PLT)in the control group and 25%group,and platelet hematocrit(PCT)in 25%,50%,and 75%groups,respectively,were significantly lower than those at the beginning,while lymphocyte ratio(LYM%)in the control group,25%,and 75%groups,as well as red blood cell(RBC)of 25%and 50%groups,were significantly increased.3)Serum aspartate transaminase(AST)and alkaline phosphatase(ALP)levels were significantly higher than normal.AST activity in the control group was significantly higher than that in the other groups,with no significant differences among the groups,and the lowest AST activity was observed in the 75%group.The ALP levels in the control group were significantly higher than those in the 50%and 100%groups,and the lowest AST activity was observed in the 100%group.albumin(ALB)and creatinine(CRE)levels in the 75%group were significantly different from those in the 25%group.There were no significant differences in other indices among the groups.4)The catalase activity(CAT)in the 50%group,total superoxide disumutase(T-SOD)activity in the 100%group,and total antioxidant capacity(T-AOC)in the 75%group were significantly higher than those in the control group.There were no significant differences in the glutathione

关 键 词：甜高粱 肉羊 血常规检查 谷草转氨酶 碱性磷酸酶 总抗氧化能力 丙二醛 

分 类 号：S826.5[农业科学—畜牧学]