梅毒血清固定患者外周血单个核淋巴细胞中miR-31和mi R-192水平表达及其诊断价值研究  

Study on the Expression Levels and Diagnostic Value of miR-31 and miR-192 in Peripheral Blood Mononuclear Lymphocytes of Patients with Syphilis Serum Fixation

作　　者：赵萍[1] 吴红霞 高海珍[2] ZHAO Ping;WU Hongxia;GAO Haizhen(Department of Dermatology,Jiangyin People’s Hospital,Jiangsu Jiangyin 214400,China;Department of Clinical Laboratory,Jiangyin People’s Hospital,Jiangsu Jiangyin 214400,China)

机构地区：[1]江阴市人民医院皮肤科,江苏江阴214400 [2]江阴市人民医院检验科,江苏江阴214400

出　　处：《现代检验医学杂志》2023年第5期29-33,46,共6页Journal of Modern Laboratory Medicine

摘　　要：目的 探讨梅毒血清固定患者外周血单个核淋巴细胞中微小核糖核酸(micro RNA,miR)-31和微小核糖核酸(micro RNA,miR)-192的表达水平及其诊断价值。方法 选取2020年11月~2022年11月江阴市人民医院收治的梅毒患者81例,根据患者进行驱梅治疗后的血清转变情况分为血清固定组(n=37)和转阴组(n=44),根据临床病理分期分为一期梅毒患者组(n=45)和二期梅毒患者组(n=36),根据梅毒快速血浆反应素试验(rapid plasma response test,RPR)分为反应素初始滴度<1∶8梅毒患者组(n=31),1∶8~1∶32梅毒患者组(n=26)和> 1∶32梅毒患者组(n=24)。另选取同期进行健康体检的50例志愿者为健康对照组。实时荧光定量PCR检测各组外周血miR-31和miR-192水平;Logistic回归分析梅毒患者发生血清固定的影响因素;受试者工作特征(receiver operating characteristic,ROC)曲线分析外周血miR-31,miR-192水平对梅毒患者发生血清固定的诊断价值。结果 血清固定组、转阴组和健康对照组外周血miR-31(0.36±0.10,0.68±0.13,1.01±0.22)和miR-192(0.51±0.12,0.73±0.19,1.03±0.05)表达水平逐渐升高,差异具有统计学意义(F=167.815,173.090,均P <0.05);病理分期为Ⅰ期患者外周血miR-31(0.56±0.13),miR-192(0.76±0.14)水平显著高于Ⅱ期(0.38±0.10,0.47±0.08),差异具有统计学意义(t=6.842,11.059,均P <0.05);反应素初始滴度<1∶8、反应素初始滴度为1∶8~1∶32、反应素初始滴度> 1∶32的梅毒患者血清miR-31(0.56±0.18,0.48±0.12,0.39±0.09)和miR-192(0.80±0.20,0.64±0.13,0.49±0.12)表达水平逐渐降低,差异具有统计学意义(F=10.057,26.166,均P <0.05);miR-31[OR(95%CI)=0.537(0.338~0.853)]和miR-192 [OR(95%CI)=0.375(0.192~0.732)]均是梅毒患者发生血清固定的保护因素(均P <0.05);外周血miR-31,miR-192以及两者联合诊断梅毒患者发生血清固定的曲线下面积(area under curve,AUC)分别为0.729(95%CI:0.618~0.821),0.821(95%CI:0.720~0.898)和0.927(95%CI:0.847~0.973),联合诊�Objective To investigate the expression level and diagnostic value of microRNA(miR)-31and microRNA(miR)-192 in peripheral blood mononuclear lymphocytes of patients with Syphilis serumfixation.Methods A total of 81 Syphilis patients admitted to Jiangyin People’s Hospital from November 2020 to November 2022 were collected.According to the serum transformation status after anti plum therapy,they were divided into a serumfixation group(n=37)and a negative conversion group(n=44).According to clinical pathological staging,there were divided into a primary Syphilis group(n=45)and a secondary Syphilis group(n=36).According to the rapid plasma response test(RPR)for Syphilis,there were divided into an initial titer<1∶8(n=31),an initial titer of 1∶8~1∶32(n=26),and an initial titer>1∶32(n=24).Another 50 volunteers who underwent physical examinations in the hospital during the same period were collected as the healthy control group.Real-timefluorescence quantitative PCR was used to detect the levels of miR-31 and miR-192 in peripheral blood of each group.Logistic regression was applied to analyze the influencing factors of serumfixation in Syphilis patients.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of peripheral blood miR-31 and miR-192 levels for serum fixation in Syphilis patients.Results The expression levels of miR-31(0.36±0.10,0.68±0.13,1.01±0.22)and miR-192(0.51±0.12,0.73±0.19,1.03±0.05)in peripheral blood of serumfixation group,negative conversion group and healthy control group were gradually increased,and the differences were statistically significant(F=167.815,173.090,all P<0.05).The levels of miR-31(0.56±0.13)and miR-192(0.76±0.14)in peripheral blood of patients with pathological stage I were significantly higher than those of patients with stage II(0.38±0.10,0.47±0.08),and the differences were statistically significant(t=6.842,11.059,all P<0.05).The levels of serum miR-31(0.56±0.18,0.48±0.12,0.39±0.09)and miR-192(0.80±0.20,0.64±0.13,0.

关 键 词：微小核糖核酸-31 微小核糖核酸-192 梅毒血清固定 

分 类 号：R377.1[医药卫生—病原生物学] R392.11[医药卫生—基础医学]