冷冻干燥口蹄疫病毒样颗粒免疫效果及生物相容性评价  

Immunological effect and biocompatibility evaluation of freeze-dried foot-and-mouth disease virus-like particles

作　　者：丁威豪 滕志东 麻玉清 董虎[2] 杨坤 宋河涛 石晓妮 孙世琪[2] 王晓强[1] 马全英 郭慧琛[2,3] DING Wei-hao;TENG Zhi-dong;MA Yu-qing;DONG Hu;YANG Kun;SONG He-tao;SHI Xiao-ni;SUN Shi-qi;WANG Xiao-qiang;MA Quan-ying;GUO Hui-chen(School of Chemistry and Chemical Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;State Key Laboratory for Animal Disease Control and Prevention,College of Veterinary Medicine,Lanzhou University,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]兰州交通大学化学化工学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室兰州大学动物医学院,甘肃兰州730046 [3]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《中国兽医科学》2023年第8期964-970,共7页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2021YFD1800300);甘肃省科技重大专项(21ZD3NA001);国家自然科学基金项目(32072859,32072847,32002272);科技人才与平台计划项目(202205AF150007);兰州交通大学-天津大学联合创新基金项目(2021059);兰州市科技计划项目(2022-2-89)。

摘　　要：为了初步探究冷冻干燥对口蹄疫病毒样颗粒(FMDV VLPs)稳定性及免疫原性的影响,采用冷冻干燥技术将FMDV VLPs疫苗抗原进行冻干处理,冷冻干燥的FMDV VLPs在保存3、6、12个月后分别免疫小鼠,检测免疫后小鼠的特异性抗体水平及淋巴细胞增殖水平;检测免疫小鼠的抗体亚型水平、细胞因子分泌水平、小鼠体重增长趋势、主要脏器指数及损伤情况等。结果显示,冷冻干燥前后FMDV VLPs的结构蛋白和粒径无明显改变;保存3、6、12月后免疫小鼠均能产生良好的特异性抗体及淋巴细胞增殖水平,抗体亚型及细胞因子分泌水平也有显著提高,且冻干前后无统计学差异;生物相容性分析显示,小鼠生长状况及主要脏器病理分析与PBS组相比无明显变化。结果表明,冷冻干燥FMDV VLPs在保存12个月后仍具有良好的免疫原性与生物相容性。To investigate the effects of freeze-drying on the stability and immunogenicity of footand-mouth disease virus like particles(FMDV VLPs).The FMDV VLPs vaccine antigen was freeze-dried using freeze-drying technology,and the mice were immunized with freeze-dried FMDV VLPs,which were stored for 3,6 and 12 months,the specific antibody and lymphocyte proliferation of the immunized mice were detected,and antibody subtypes,cytokine secretion,body weight,the index and injury of major organ were also measured.The results showed that the characteristics of FMDV VLPs did not change significantly before and after freeze-drying.After storage for 3,6 and 12 months,the immunized mice could produce high level of specific antibody and lymphocyte proliferation,and the secretion of antibody subtypes and cytokine were also significantly improved,and there was no statistical difference before and after lyophilization.Biocompatibility analysis results showed that there was no significant change in the growth status of mice and no pathological change in major organs compared with control group.This study confirmed that freeze-dried FMDV VLPs still have high immunogenicity and biocompatibility after 12 months of storage.

关 键 词：口蹄疫 病毒样颗粒 冷冻干燥 免疫效果 生物相容性 

分 类 号：S852.659.6[农业科学—基础兽医学]