甘肃省部分地区绵羊膈肌住肉孢子虫的感染情况调查与分子生物学鉴定  

Investigation of infection and molecular biological identification of Sarcocystis spp.in the diaphragm of sheep from parts of Gansu Province

作　　者：孙继文 游杰 尚小粉 王贺 李媛媛 宋蓓蓓 贾万忠[2] 马永华 SUN Ji-wen;YOU Jie;SHANG Xiao-fen;WANG He;LI Yuan-yuan;SONG Bei-bei;JIA Wan-zhong;MA Yong-hua(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;State Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院,兰州兽医研究所,动物疫病防控全国重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2023年第6期757-763,共7页Chinese Veterinary Science

基　　金：甘肃省自然科学基金项目(18JR3RA167);兰州市科技计划项目(2022-2-112,2017-4-103);甘肃省优秀研究生“创新之星”项目(2022CXZXS-010)。

摘　　要：为调查甘肃省绵羊膈肌住肉孢子虫的感染情况,自兰州市永登县、临夏回族自治州东乡族自治县、定西市陇西县、白银市靖远县采集绵羊膈肌样品188份,以住肉孢子虫cox1、18S rRNA和ITS1为靶基因对所采集的样品进行PCR检测,并将阳性样品进行测序。结果显示,基于cox1、18S rRNA和ITS1基因PCR检测的住肉孢子虫总感染率分别为42.55%(80/188)、26.06%(49/188)和37.77%(71/188),3种检测方法均以东乡族自治县的感染率最高。基于cox1基因的检测中,有8份采集自东乡族自治县的阳性样品为公羊犬住肉孢子虫,其余72份阳性样品为柔嫩住肉孢子虫;基于18S rRNA基因的检测中,有1份采集自东乡族自治县的阳性样品为公羊犬住肉孢子虫,其余48份阳性样品为柔嫩住肉孢子虫;基于ITS1基因的检测中,71份阳性样品均为柔嫩住肉孢子虫。结果表明,基于3个基因的PCR方法中,以cox1为靶基因的PCR方法有更高的灵敏度、特异性和更好的虫种鉴定能力。本研究为甘肃省绵羊住肉孢子虫病分子流行病学调查和虫种鉴定研究补充了基础数据,为住肉孢子虫病的诊断和防控提供了参考依据。In order to investigate the infection of Sarcocystis spp.in the diaphragm of sheep from Gansu Province,188 diaphragm samples of sheep were collected from Yongdeng County of Lanzhou City,Dongxiang Autonomous County of Linxia Hui Autonomous Prefecture,Longxi County of Dingxi City and Jingyuan County of Baiyin City.The samples were detected by PCR based on cox1 gene,18S rRNA gene and ITS1 gene of Sarcocystis spp..The positive samples were sequenced.The results showed that the total infection rates of Sarcocystis spp.detected by PCR based on cox1,18S rRNA and ITS1 gene were 42.55%(80/188),26.06%(49/188)and 37.77%(71/188),respectively.The highest infection rate was in Dongxiang Autonomous County based on both 3 methods of detection.In the detection based on cox1,8 positive samples collected from Dongxiang Autonomous County were S.arieticanis,and other 72 positive samples were S.tenella.In the detection based on 18S rRNA,1 positive sample collected from Dongxiang Autonomous County was S.arieticanis,and other 48 positive samples were S.tenella.In the detection based on ITS1 gene,all 71 positive samples were S.tenella.The results indicated that the PCR based on cox1 gene had higher sensitivity,specificity and better species identification ability among the PCR based on 3 genes.This study filled the basic data in the molecular epidemiology and species identification of sarcocystosis in sheep from Gansu Province and provided a reference basis for the diagnosis and control of sarcocystosis.

关 键 词：绵羊 住肉孢子虫 感染情况调查 分子生物学鉴定 

分 类 号：S852.723[农业科学—基础兽医学]