羟氯扎胺盐酸左旋咪唑复方混悬液对靶动物羊的安全性评价  

Target animal safety of oxyclozanide and levamisole hydrochloride compound suspension in sheep

作　　者：陈玲玲 张东辉[1] 姚学强 高馨儿 周凯仁 张继瑜[1] 周绪正[1] CHEN Ling-ling;ZHANG Dong-hui;YAO Xue-qiang;GAO Xin-er;ZHOU Kai-ren;ZHANG Ji-yu;ZHOU Xu-zheng(Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture and Rural Affairs,Key Laboratory of New Animal Drug Project of Gansu Province,Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China)

机构地区：[1]中国农业科学院、兰州畜牧与兽药研究所、农业农村部兽用药物创制重点实验室、甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《中国兽医科学》2023年第6期772-779,共8页Chinese Veterinary Science

基　　金：国家肉牛牦牛产业技术体系项目(CARS-37);临潭县肉牛产业提质增效关键技术集成与示范项目(2022YED1602201)。

摘　　要：为研究羟氯扎胺盐酸左旋咪唑复方混悬液对靶动物羊的安全性,选取32只(雌雄各半)体重在20 kg左右的健康小尾寒羊,随机分成4组,每组8只。分别为1倍(0.25 mL/kg)剂量组、3倍(0.75 mL/kg)剂量组、5倍(1.25 mL/kg)剂量组和对照组,给药组逐只按量经口灌服给药,每天用药1次,连用3 d;对照组灌服等体积生理盐水。给药后连续观测14 d,每天观察试验羊的精神状况、饮食欲及二便,检测体温等。试验期间除进行一般临床健康检查状况观察以外,试验前、给药后及观测期结束还进行增重、血常规和血清生化指标检测,对对照组和5倍剂量组进行大体病理学和组织病理学检查。结果显示,试验组羊在给药期间出现不同程度的腹泻和食欲不振,给药结束后症状逐渐缓解;试验组与对照组相比,血液学检查和血清生化指标结果未见明显差异;大体剖检未有显著的病理变化;与空白剂量组相比,5倍剂量组羊的组织病理学检查也未见明显异变,未出现中毒及死亡现象。上述结果表明,羟氯扎胺盐酸左旋咪唑复方混悬液在以5倍剂量给药时对靶动物仍安全,通过靶动物安全性试验,为羟氯扎胺盐酸左旋咪唑复方混悬液的安全性评价及临床科学用药提供了依据。To investigate the safety of oxyclozanide and levamisole hydrochloride compound suspension on target animals of sheep.32 healthy Ovis aries weighing about 20 kg were randomly divided into 4 groups with 8 animals in each group,which were the 1 time(0.25 mL/kg),3times(0.75 mL/kg),5 times(1.25 mL/kg)clinically recommended dose groups and the control group.The dosing group was administered orally by instillation once a day for 3 days,the control group was administered saline of equal volume.The sheep were continuously observed for 14 days,and the mental state,appetite,stool and body temperature were observed every day.During the test,in addition to the observation of general clinical health examination,weight gain,blood routine test and serum biochemical indicators were also detected before test,after the administration and at the end of the observation period,and gross pathology and histopathology were examined for the control group and the five times dose group.The results showed that the experimental group of sheep experienced varying degrees of diarrhea and loss of appetite during the administration period,and symptoms gradually relieved after the administration was completed.There was no significant difference in hematological examination and serum biochemical indicators between the experimental group and the control group,no significant pathological changes were found in gross autopsy.Compared with the control group,the histopathological examination of the sheep in the 5-fold dose group also showed no obvious changes,no poisoning and death.The oxyclozanide and levamisole hydrochloride compound suspension is still safe for target animals when it is given at 5 times the dose.The target animal safety test provided a basis for the safety evaluation and clinical scientific use of the compound suspension of levamisole hydrochloride.

关 键 词：羟氯扎胺 盐酸左旋咪唑 复方混悬液 羊 安全性评价 

分 类 号：S859.795[农业科学—临床兽医学]