基于生长周期对仙茅根茎及须根中仙茅苷积累的影响研究  

Study on the effect of growth cycle on the accumulation of Curculigoside in Rhizomes and Fibrous root of Curculigo orchioides Gaertn.

作　　者：朱新焰[1] 杨天梅[1] 起明菊 杨美权[1] 杨维泽[1] 许宗亮[1] 冯人和 李绍兴 张金渝[1] ZHU Xin-yan;YANG Tian-mei;QI Ming-ju;YANG Mei-quan;YANG Wei-ze;XU Zong-liang;FENG Ren-he;LI Shao-xing;ZHANG Jin-yu(Institute of Medicinal Plant,Yunnan Provincial Academy of Agricultural Sciences,Kunming 650205,China;Lincang Institute of Science and Technology Information,Lincang Yunnan 677000,China;Linxiang District Local Industrial Development Service Center,Lincang Yunnan 677000,China)

机构地区：[1]云南省农业科学院药用植物研究所,云南昆明650205 [2]临沧市科学技术情报研究所,云南临沧677000 [3]临沧市临翔区地方产业发展服务中心,云南临沧677000

出　　处：《时珍国医国药》2023年第6期1470-1472,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家可持续发展示范区科技专项(临沧)(202104AC100001-A13);云南省2021年重大科技专项(生物医药)(202102AA100013);国家自然科学基金(31960391)。

摘　　要：目的:探讨不同生长周期的仙茅根茎中标志物仙茅苷的积累规律,同时测定多年生仙茅须根中仙茅苷的含量,为仙茅质量控制和资源充分开发利用提供依据。方法采用HPLC法,以ACE Excel5 C18-AR柱(5m,4.6 mm×250 mm)为色谱柱,以以乙腈-0.1%磷酸溶液(21:79)进行等度洗脱,流速:1.0ml/min;检测波长:285nm,测定各样品仙茅苷的含量。结果经过对HPLC图谱分析比较,不同生长年限的仙茅中仙茅苷含量差别较大,以4年生最高。3年生的次之,1年生则最低。3~4年生仙茅须根中仙茅苷平均含量达到0.390%~0.425%,4年生含量略高。结论3~4年生仙茅根茎的仙茅苷含量均达到《中国药典》(2020年版)规定,以3年及以上生长年限的仙茅作为药材原料具有一定的合理性。同时,3年及以上生长年限的仙茅须根的仙茅苷含量已经远超出了相同生长年限的根茎,仙茅须根可进一步开展资源评价,以便充分开发利用资源。Objective To explore the accumulation of Curculigoside in the rhizome of Curculigo orchioides in different growth pe-riods,and to determine the content of curculigoside in the fibrous root of Curculigo orchioides,so as to provide a basis for quality control and full development and utilization of resources of citronella.Methods The chromatographic separation was performed on ACE Excel5 C18-AR(5m,4.6×250mm)column.The mobile phase consisting of acetonitrile-phosphoric acid(0.1%)solu-tion(21:79)in gradient elution.The flow rate was 1.0 mL·min-1,and the detection wavelength was 285 nm.Results Through the analysis and comparison of HPLC spectra,the content of curculigoside in different growth years was different.The growth of 4 years is the highest,the growth of 3 years is the second,and the growth of 1 year is the lowest.Conclusion The content of curcu-ligoside in the rhizome of 3-4-year-old citronellae can meet the requirements of the Chinese Pharmacopoeia(2020 Edition),and it is reasonable to use Curculigo orchioides with 3-year and above growth years as the raw material of medicinal materials.At the same time,the content of curculigoside in the fibrous roots of Curculigo orchioides with 3 years and above growth years has far exceeded that of the rhizomes with the same growth years.Further resource evaluation can be carried out for the fibrous roots of Curculigo orchioides in order to fully develop and utilize the resources.

关 键 词：生长周期 仙茅 根茎 须根 仙茅苷 HPLC 

分 类 号：S567[农业科学—中草药栽培]