应用于二氧化硫检测及活细胞成像的荧光增强型探针  

A fluorescence enhanced probe for sulfur dioxide detection and fluorescence imaging in living cells

作　　者：王敏 张静怡 刘娜 张迪 WANG Min;ZHANG Jingyi;LIU Na;ZHANG Di(College of Chemistry and Chemical Engineering,Zhengzhou Normal University,Zhengzhou 450044,China;Institute of Agricultural Quality Standards and Testing Technology,Henan Academy of Agricultural Sciences,Zhengzhou 450052,China)

机构地区：[1]郑州师范学院化学化工学院,郑州450044 [2]河南省农业科学院农业质量标准与检测技术研究所,郑州450052

出　　处：《分析试验室》2023年第8期1094-1099,共6页Chinese Journal of Analysis Laboratory

基　　金：河南省科技攻关项目(222102310384);郑州师范学院科研启动专项经费(702446)资助。

摘　　要：基于香豆素和苯并吡啶基团,构建了用于二氧化硫(SO_(2))高效检测的荧光探针P1,其化学结构通过核磁氢谱(^(1)H NMR)、碳谱(^(13)C NMR)和高分辨质谱(HR-MS)确证。在缓冲溶液体系中,单独的探针P1具有微弱的荧光,识别SO_(2)后荧光发射强度明显增强,能够实现对SO_(2)的专一性裸眼识别,检出限为126 nmol/L。生物应用实验结果表明,该探针具有较低的细胞毒性,可用于生物活细胞中外源性SO_(2)的荧光成像。A fluorescent probe P1 was constructed for efficient detection of sulfur dioxide(SO_(2))based on coumarin and benzopyran groups.Its chemical structure was confirmed by hydrogen-nuclear magnetic resonance(^(1)H NMR),^(13)carbon-nuclear magnetic resonance(^(13)C NMR)and high resolution mass spectrometry(HR-MS).In buffer solution system,the single probe P1 showed very weak fluorescence,and the fluorescence emission intensity significantly enhanced after the recognition of SO_(2),which could realize the specific naked eye recognition of SO_(2)with the detection limit of 126 nmol/L.The results of biological application experiments indicated that the probe had low cytotoxicity and could be used for fluorescence imaging of exogenous SO_(2)in living biological cells.

关 键 词：二氧化硫 荧光探针 荧光增强 荧光成像 

分 类 号：O657.3[理学—分析化学]