二维液相色谱阀切换接口技术的发展及应用  

Development and application of valve interface technology in two-dimensional liquid chromatography

作　　者：姜艺菲 赵学佳 山广志 JIANG Yifei;ZHAO Xuejia;SHAN Guangzhi(Analysis and Testing Center of Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences,Beijing 100050,China)

机构地区：[1]中国医学科学院医药生物技术研究所分析测试中心,北京100050

出　　处：《分析试验室》2023年第8期1127-1136,共10页Chinese Journal of Analysis Laboratory

摘　　要：在复杂样本分析中,一种分离模式往往不能提供足够的分离度,组合不同的分离模式构建二维液相色谱系统是解决这一问题的有效途径。阀切换接口是二维液相色谱柱切换技术的核心,接口技术影响系统的分离度和灵敏度。传统的接口技术在生物、药学、食品、环境等领域得到了广泛应用,近20年发展了众多新型的接口技术来解决二维液相色谱中正交组合溶剂不兼容问题。本文综述了柱切换接口技术的原理和应用,并总结了近5年利用柱切换二维液相分析技术的进展,期望为二维液相色谱技术的发展和应用提供借鉴。One separation mode usually cannot provide sufficient resolution in the analysis of complex samples.Combining different separation modes to construct two-dimensional liquid chromatography(2D-LC)system is an effective way to solve this problem.The key of 2D-LC system is the valve switching interface which affects the separation and sensitivity.In the past 20 years,traditional interface techniques has been widely used in biology,pharmacy,food and environment fields,and many new interface techniques have been developed to solve the problem concerning the incompatibility of orthogonal solvents.This paper reviews the principles and applications of column switching interface technology and summarizes the literatures published in the last five years,hoping to provide reference for the development and application of 2D-LC system in the future.

关 键 词：二维液相色谱 阀/柱切换接口 复杂样品分析 

分 类 号：O657.7[理学—分析化学] O652.63[理学—化学]