HPLC⁃DAD法同时测定当归祛风合剂中6种成分  

Simultaneous determination of six constituents in Danggui Qufeng Mixture by HPLC-DAD

作　　者：谢寒 明荷[2] 方应权[2] 祝守敏 XIE Han;MING He;FANG Ying-quan;ZHU Shou-min(Affiliated Hospital of Chongqing Three Gorges Medical College,Chongqing 404000,China;Chongqing Three Gorges Medical College,Chongqing 404120,China)

机构地区：[1]重庆三峡医药高等专科学校附属医院,重庆404000 [2]重庆三峡医药高等专科学校,重庆404120

出　　处：《中成药》2023年第9期2842-2846,共5页Chinese Traditional Patent Medicine

基　　金：重庆市教委科学技术研究项目(KJQN202002713);重大自然科学课题(2018zdxz01)。

摘　　要：目的建立HPLC-DAD法同时测定当归祛风合剂中阿魏酸、毛蕊花糖苷、芍药苷、白鲜碱、升麻素苷、甘草苷的含量。方法该药物50%甲醇提取液的分析采用Ultimate C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相0.1%磷酸-乙腈,梯度洗脱;体积流量1.0 mL/min;柱温35℃;检测波长230、236、240、280、334 nm。结果6种成分在各自范围内线性关系良好(r>0.9994),平均加样回收率95.56%~105.29%,RSD 2.77%~3.92%。结论该方法准确可靠,可为当归祛风合剂质量控制、临床应用提供参考。AIM To establish an HPLC-DAD method for the simultaneous content determination of ferulic acid,acteoside,peoniflorin,dictamnine,prim-O-glucosylcimifugin,and liquiritin in Danggui Qufeng Mixture.METHODS The analysis of 50%methanol extract of this drug was performed on a 35℃thermostatic Ultimate C_(18) column(250 mm×4.6 mm,5μm),with the mobile phase comprising of 0.1%phosphoric acid-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 230,236,240,280,334 nm.RESULTS Six constituents showed good linear relationships within their own ranges(r>0.9994),whose average recoveries were 95.56%-105.29%with the RSDs of 2.77%-3.92%.CONCLUSION This stable and reliable method can provide a reference for the quality control and clinical application of Danggui Qufeng Mixture.

关 键 词：当归祛风合剂 阿魏酸 毛蕊花糖苷 芍药苷 白鲜碱 升麻素苷 甘草苷 HPLC 

分 类 号：R927.2[医药卫生—药学]