淫羊藿苷对尾悬吊模拟失重诱导大鼠骨丢失的防护作用  

Protective effects of icariin on bone loss induced by tail-suspension simulated weightlessness in rats

作　　者：白亚楠 万美玉 狄晓可 张志斐 李小娜 李洋 BAI Ya-nan;WAN Mei-yu;DI Xiao-ke;ZHANG Zhi-fei;LI Xiao-na;LI Yang(School of Pharmacy,North China University of Science&Technology,Tangshan 063210,China)

机构地区：[1]华北理工大学药学院,河北唐山063210

出　　处：《中成药》2023年第9期2890-2895,共6页Chinese Traditional Patent Medicine

基　　金：河北省自然科学基金项目(H2021209059,H2016209319);华北理工大学博士科研启动基金项目(25759799)。

摘　　要：目的研究淫羊藿苷对尾悬吊模拟失重大鼠骨丢失的影响。方法大鼠随机分为空白组、模型组、阿仑膦酸钠组(2.0 mg/kg)、淫羊藿苷组(30 mg/kg),每组10只。大鼠灌胃给药6周,每周称重1次,从第3周开始,除空白组外,其他各组均给予尾悬吊处理。6周后,ELISA法检测大鼠相关骨代谢标志物水平;双能X线骨密度仪检测大鼠骨密度;三点弯曲力学实验分析大鼠股骨生物力学性能;Micro-CT扫描仪观察大鼠股骨形态结构特性并对骨小梁进行三维重建,测定相关空间结构参数;Schrodinger Maestro 12.8软件将阿仑膦酸钠、淫羊藿苷分别与BMP-2、OPG、RANK、RANKL蛋白进行分子对接;Western blot法检测股骨组织BMP-2、OPG、RANK、RANKL蛋白表达。结果与模型组比较,淫羊藿苷组骨吸收相关标志物水平降低(P<0.05,P<0.01),骨形成相关标志物水平、骨密度、股骨骨应力、弹性模量及最大载荷、股骨组织BMP-2和OPG蛋白表达升高(P<0.05,P<0.01);淫羊藿苷较阿仑膦酸钠与骨代谢蛋白对接更好,更易发生作用。结论淫羊藿苷可有效防护尾悬吊模拟失重引起的大鼠骨丢失,具有良好的研究前景。AIM To study the effects of icariin on bone loss in rats by tail-suspension simulated weightlessness.METHODS The rats randomly divided into the blank group,the model group,the alendronate group(2.0 mg/kg)and the icariin group(30 mg/kg),with 10 rats in each group were given 6 weeks intragastric administration and weighet measurement once a week.Exposure to tail-suspension treatment started on the third week in all groups except the blank group,and concluded 6 weeks later.And then,the rats had their levels of related bone metabolic markers detected by ELISA;their bone mineral density(BMD)detected by dual-energy X-ray absorptiometry;their femoral biomechanical properties analyzed by three-point bending mechanics experiment;and their femoral morphological and structural characteristics observed by Micro-CT scanner,their trabecular bone three dimensional reconstructed,and the related spatial structural parameters determined.Schr dinger Maestro 12.8 software made molecular docking of alendronate sodium and icariin with BMP-2,OPG,RANK and RANKL proteins respectively.Western blot was used to detect the protein expressions of BMP-2,OPG,RANK and RANKL in rat femur.RESULTS Compared with the model group,the icariin group shared decreased level of bone resorption-related markers(P<0.05,P<0.01);and increased level of bone formation-related markers,bone density,femoral bone stress,elastic modulus and maximum load,and protein expressions of BMP-2 and OPG in femoral tissue(P<0.05,P<0.01).With more inclination to act,icariin displayed better docking with bone metabolic proteins than alendronate sodium.CONCLUSION Icariin-exerted preventive effects on bone loss induced by tail-suspension simulated weightlessness in rats highlight its future research prospect.

关 键 词：淫羊藿苷 尾悬吊 骨丢失 骨代谢 骨吸收 

分 类 号：R285.5[医药卫生—中药学]