ELISA实验中微量加样量对检测丙肝抗体的影响评价  

Evaluation of the effect of micro sample addition on the detection of hepatitis C antibodies in ELISA experiments

作　　者：陈文花 Chen Wenhua(Disease Prevention and Control Center of Xihu District,Nanchang,Jiangxi 330000,China)

机构地区：[1]南昌市西湖区疾病预防控制中心,江西南昌330000

出　　处：《首都食品与医药》2023年第18期87-89,共3页Capital Food Medicine

摘　　要：目的研究ELISA实验中加样量变化对于检测丙肝抗体结果的影响。方法于南昌市西湖区疾病预防控制中心2021年3月-2022年10月检测的不同浓度的血清样本中随机抽取5份,使用不同加样量进行测定,计算S/C0值。结果1号和5号样本随着加样量增加,S/CO值结果变化不大,即随着加样量增加,S/CO值变化不存在线性关系。2号、3号和4号样本随着加样量增加,S/CO值也呈现明显升高趋势,即随着样本加样量增加,S/CO值存在一定正相关关系。在3号样本中,加样量不足(≤8μL)时,S/CO值<1,为阴性。当S/CO值在加样量<6μL和>14μL时,加样量变化对于S/CO结果无明显影响。结论为了保证ELISA方法测定丙肝抗体检测结果的准确性,在实验过程中要严格遵守技术要求,使用稳定试剂以及先进设备,更需要保证加样量的准确值,严格按照规范操作进行加样处理,避免假阳性以及假阴性的结果。Objective To study the impact of changes in sample size on the results of detecting hepatitis C antibodies in ELISA experiments.Method Randomly select 5 serum samples of different concentrations tested in our center from March 2021 to October 2022,using different sample sizes for measurement,calculate the S/CO value.Result The S/CO values of samples 1 and 5 showed litle change with the increase of sample volume,and there was no linear relationship between the S/CO values and the increase of sample volume.The S/CO values of samples 2,3,and 4 also showed a significant upward trend as the sample size increased,and there was a certain positive correlation between the S/CO values as the sample size increased.In sample number 3,insufficient sample addition(≤8μL)when the S/CO value is less than 1,it is negative.When the S/CO value is less than 6μL and>14μL,the change in sample size has no significant impact on the S/CO results.Conclusion In order to ensure the accuracy of the ELISA method for detecting hepatitis C antibody detection results,it is necessary to strictly comply with technical requirements,use'stable reagents and advanced equipment during the experimental process,and ensure the accurate value of the sample amount.The sample addition process should be strictly carried out in accordance with standard operations to avoid false positive and false negative results.

关 键 词：丙肝抗体检测 ELISA实验 加样量 

分 类 号：R446[医药卫生—诊断学]