胎鼠真皮间充质干细胞对小鼠巨噬细胞极化的影响  

Effect of Fetal Dermal Mesenchymal Stem Cells on Mouse Macrophage Polarization

作　　者：夏震宇 龚思宇 王晓川 姜笃银 单菲[4] 赵洁[4] XIA Zhenyu;GONG Siyu;WANG Xiaochuan;JIANG Duyin;SHAN Fei;ZHAO Jie(Cheeloo College of Medicine,Shandong University,Jinan 250012;School of Basic Medicine,Jining Medical College,Jining 272067,Shandong,China;Department of Burns and Plastic Surgery,the Second Hospital of Shandong University,Jinan 250033,Shandong,China;Department of Emergency,the Second Hospital of Shandong University,Jinan 250033,Shandong,China)

机构地区：[1]山东大学齐鲁医学院,山东济南250012 [2]山东省济宁医学院基础医学院,山东济宁272067 [3]山东大学第二医院整形烧伤科,山东济南250033 [4]山东大学第二医院急诊医学中心,山东济南250033

出　　处：《中国美容医学》2023年第9期58-60,77,共4页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(编号:81873934);王正国创伤医学发展基金会“生长因子复兴计划”(编号:SZYZ-TR-09);山东大学临床研究中心急危重症重点专项(编号:2021SDUCRCA008);山东省济宁医学院基础医学院项目(编号:CX2020038)。

摘　　要：目的:探究胎鼠真皮间充质干细胞(Fetal mice dermal mesenchymal stem cells,FDMSCs)对M1/2型巨噬细胞极化的影响。方法:本研究提取FDMSCs,利用脂多糖(Lipopolysaccharide,LPS)、γ干扰素(Interferon-γ,IFN-γ)刺激RAW 264.7诱导为M1型巨噬细胞,利用白细胞介素4(Interleukin-4,IL-4)刺激RAW264.7诱导为M2型巨噬细胞,并建立了FDMSCs-RAW 264.7共培养体系。共培养24 h后,收集巨噬细胞,用实时定量PCR和流式细胞学检测M1/2型巨噬细胞特征性因子白细胞介素6(Interleukin-6,IL-6)、诱导性一氧化氮合酶(Inducible nitric oxide synthase,iNOS)、CD86、白细胞介素10(Interleukin-10,IL-10)、精氨酸酶1(Arg-1)、CD206的表达变化。结果:FDMSCs使M1型巨噬细胞表达的IL-6、iNOS、CD86减少,IL-10、CD206增多;使M2型巨噬细胞表达的IL-10、Arg-1、CD206表达增多,iNOS表达减少。结论:FDMSCs可以通过调控巨噬细胞极化来调控伤口愈合,对于探索FDMSCs在创面治疗过程中的免疫调节机制有积极意义。Objective To investigate the effect of FDMSCs on the polarisation of M1/2 type macrophages.Methods In this study,RAW264.7 was induced into M1-type macrophages by stimulation with lipopolysaccharide(LPS)and gamma interferon(IFN-γ)and into M2-type macrophages by stimulation with interleukin 4(IL-4),and a FDMSCs-RAW264.7 co-culture system was established.After 24 hours of co-culture,macrophages were harvested and changes in the expression of the characteristic factors interleukin 6(IL-6),inducible nitric oxide synthase(iNOS),CD86,interleukin 10(IL-10),arginase 1(Arg-1)and CD206 in M1/2 type macrophages were detected by qRT-PCR and flow cytometry.Results FDMSCs decreased the expression of IL-6,iNOS and CD86 and increased the expression of IL-10 and CD206 in M1 macrophages;increased the expression of IL-10,Arg-1 and CD206 and decreased the expression of iNOS in M2 macrophages.Conclusion FDMSCs can regulate wound healing by modulating macrophage polarisation,which has positive implications for exploring the immunomodulatory mechanisms of FDMSCs in the process of wound treatment.

关 键 词：胎鼠 真皮间充质干细胞 巨噬细胞极化 M1/M2亚型 创面愈合 共培养 

分 类 号：R751[医药卫生—皮肤病学与性病学]