江南星蕨化学成分的分离与鉴定  

Isolation and identification of chemical constituents from Lepisorus fortunei

作　　者：吴清源 汪丽丽 黄国正 王全喜[1,3] 戴锡玲[1] 曹建国[1] WU Qingyuan;WANG Lili;HUANG Guozheng;WANG Quanxi;DAI Xiling;CAO Jianguo(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;College of Chemistry and Chemical Engineering,Anhui University of Technology,Ma’anshan 243002,Anhui,China;Shanghai Key Laboratory of Plant Functional Genomics and Resource,Shanghai Chenshan Botanical Garden,Shanghai 201602,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]安徽工业大学化学化工学院,安徽马鞍山243002 [3]上海辰山植物园,上海资源植物功能基因组重点实验室,上海201602

出　　处：《上海师范大学学报（自然科学版）》2023年第4期424-431,共8页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海植物种质资源工程技术研究中心项目(17DZ2252700)。

摘　　要：采用硅胶、Sephadex LH-20、中压、半制备高效液相和其他色谱技术对江南星蕨(Lepisorus fortunei)的化学成分进行分离,通过理化性质和核磁共振(NMR)数据鉴定化合物的结构.从90%(体积分数)乙醇提取物中分离得到15个化合物,分别鉴定为反式咖啡酸(1)、反式乌毛蕨酸(2)、迷迭香酸(3)、5-O-咖啡酰莽草酸(4)、(R)-3-(3,4-dihydroxyphenyl)-2Rlactic acid(5)、polybotrin(6)、苏铁蕨酸(7)、羊齿烯(8)、羊齿烯酸(9)、β-谷甾醇(10)、胡萝卜苷(11)、尿嘧啶(12)、豆甾-4-烯-3,6-二酮(13)、麦角甾-4,6,8,22-四烯-3-酮(14)和牡荆素(15).其中,化合物2、4、5、7、9、13、14和15为首次从江南星蕨植物中分离得到.本研究为阐明江南星蕨药用价值提供了化学基础.The chemical constituents of Lepisorus fortunei(T.Moore)Li Wang were separated and purified by silica gel,Sephadex LH-20,MPLC,semi-preparative HPLC and other chromatographic techniques,and the structures of the compounds were identified by their physicochemical properties and nuclear magnetic resonance(NMR)data.Fifteen compounds were isolated from 90%(volume fraction)ethanolic extract and identified as trans-caffeic acid(1),(-)-trans blechnic acid(2),rosmarinic acid(3),5-O-caffeoylshikimic acid(4),(R)-3-(3,4-dihydroxyphenyl)-lactic acid(5),polybotrin(6),(-)-trans-brainic acid(7),fern-9(11)-ene(8),fern-9(11)-en-28-oic acid(9),β-sitosterol(10),daucosterol(11),uracil(12),stigmast-4-ene-3,6-dione(13),(22E,24R)-ergosta-4,6,8,22-tetraen-3-one(14)and vitexin(15).Compounds 2,4,5,7,9,13,14 and 15 were isolated for the first time from the L.fortunei.The present investigation provided chemical basis for clarifying the medicinal value of L.fortunei.

关 键 词：江南星蕨 化学成分 结构鉴定 

分 类 号：Q946.8[生物学—植物学]