^(60)Co-γ射线辐照对月季“蜻蜓”的诱变效应及突变体ISSR分析  

^(60)Co-γ mutagenic effect of irradiation on Chinese rose variety Dragonfly and analysis of mutant ISSR

作　　者：夏萌 左悠 杨碧楠 李博文 明凤 XIA Meng;ZUO You;YANG Binan;LI Bowen;MING Feng(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Shenghua Zizhu College,Shanghai 200241,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]上海圣华紫竹学院,上海200241

出　　处：《上海师范大学学报（自然科学版）》2023年第4期443-452,共10页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市花卉产业体系建设项目(沪农科产字(2022)第8号)。

摘　　要：为培育花色独特、栽培品质优良的新型蓝色月季,使用^(60)Co-γ射线对月季“蜻蜓”进行辐照处理,并设置40,50,60 Gy三个剂量梯度.月季“蜻蜓”辐照产生的诱变效应表现为:(1)辐照处理均会对植株造成一定的损伤和产生抑制生长效应,且高剂量辐照处理的植株在生长过程中,出现生长点或老叶叶尖枯死、新叶生长缓慢、新芽不再萌发、植株根部萎蔫且出现腐烂发黑现象;(2)突变体在花型、花色、花期、花瓣等花部形状上与对照组(CK)存在较大差异,具体表现在突变体的最大花径增大或缩小、花色改变、花期缩短、花瓣数量和花瓣形态改变.采用简单重复序列区间扩增多态(ISSR)分子标记技术及非加权组平均法(UPGMA)对辐照处理的月季“蜻蜓”材料进行遗传多样性和亲缘关系聚类分析,发现月季“蜻蜓”经过辐照诱变之后遗传物质有不同程度的突变.以上结果为培育优质蓝色月季品种奠定了基础.In order to cultivate a new type of blue rose with unique flower color and excellent cultivation quality,the radiation of ^(60)Co-γwas used irradiating the Chinese rose variety Dragonfly,and three doses of 40,50 and 60 Gy were set.The study found that the mutagenesis caused by the irradiation of Chinese rose Dragonfly is reflected in:firstly,the irradiation treatment has a certain damage and growth inhibition effect on the plants,and the growth points or old leaf tips of the plants treated with high-dose irradiation die during the growth process,the new leaves grow slowly,the new buds do not germinate again,and the plant roots wilt and rot and blacken.Secondly,the mutant has great differences with control check(CK)in flower shape,flower color,flowering period,petal and so on,which is specifically reflected in the increase or decrease of the maximum flower diameter,the change of flower color,the shortening of flowering period,the change of petal number and petal morphological characteristics.The genetic diversity and genetic relationship of irradiated the Dragonfly were analyzed by inter-simple sequence repeat(ISSR)molecular marker and unweighted pair-group method with airthmetic(UPGMA)cluster analysis.It was found that the genetic material of the Dragonfly had different degrees of mutation after mutational irradiations.The results laid a foundation for cultivating high-quality blue rose varieties.

关 键 词：^(60)Co-γ射线 物理诱变 简单重复序列区间扩增多态(ISSR) 分子育种 月季 

分 类 号：Q94.3[生物学—植物学]