地下水原位修复过程中微生物群落结构的变迁  

Changes of microbial community in structure during remediation of groundwater system in-situ

作　　者：邹莎莎 ZOU Shasha(Shanghai Textile Architectural and Research Institute Co.,Ltd.,Shanghai 200082,China)

机构地区：[1]上海纺织建筑设计研究院有限公司,上海200082

出　　处：《上海师范大学学报（自然科学版）》2023年第4期486-494,共9页Journal of Shanghai Normal University(Natural Sciences)

摘　　要：针对主要受三氯乙烯(TCE)和二氯乙烯(DCE)污染的地下水,将复合药剂注入地下,在缺氧/厌氧条件下,经30 d的修复,可以使TCE完全去除,同时使DCE浓度降至风险控制值以下.注药前,地下水中变形菌门(Proteobacteria)的相对丰度高达95.44%.注入复合药剂后,厚壁菌门(Firmicutes)的相对丰度增至53.45%.同时,具有TCE和DCE降解功能的梭菌属(Clostridium)和毛球菌属(Trichococcus)在复合药剂的刺激下富集,其相对丰度从0分别增加到35.17%和14.00%,成为了新的优势菌属,使受污染的地下水得到有效修复.热图分析表明,复合药剂对TCE和DCE降解微生物的生长繁殖起到了促进作用.通过分析地下水中微生物群落结构的变化可以间接评估地下水的修复效果.For groundwater mainly contaminated with trichloroethylene(TCE)and dichloroethylene(DCE),a composite agent was injected in the ground to remediate it under anoxic/anaerobic conditions for 30 d,during which the TCE was completely disappeared and the DCE concentration decreased to less than the risk control value.Before injecting composite agents,the abundance of phylum Proteobacteria in groundwater reached at 95.44%.However,after injecting the composite agents,the abundance of phylum Firmicutes increased to 53.45%.Correspondingly,genera Clostridium and Trichococcus,which can degrade TCE and DCE,were enriched due to be stimulated by the composite agents,and their abundance increased to 35.17%and 14.00%from zero,respectively,becoming new dominant genera,by which the contaminated groundwater was effectively remedied.Analysis of heat map showed that the addition of composite agents promoted the growth of TCE and DCE degrading bacteria.Changes of microbial community in structure reflected indirectly the effect of groundwater remediation.

关 键 词：地下水修复 三氯乙烯(TCE) 二氯乙烯(DCE) 微生物群落 

分 类 号：X523[环境科学与工程—环境工程]