灵武长枣叶片阿拉伯半乳糖蛋白分布特征  

Distribution characteristics of arabinogalactan protein in leavesof Ziziphus jujuba Mill cv. Lingwuchangzao

作　　者：陶珊珊 王静[1] 章英才[1] 白琳 刘迎雪 TAO Shanshan;WANG Jing;ZHANG Yingcai;Bai Lin;LIU Yingxue(School of Life Sciences,Ningxia University,Yinchuan,Ningxia 750021,China)

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021

出　　处：《干旱地区农业研究》2023年第5期22-31,50,共11页Agricultural Research in the Arid Areas

基　　金：国家级大学生创新创业训练计划项目(G202210749023);宁夏自然科学基金(2022AAC03087)。

摘　　要：以膨大前期、快速膨大期、着色期和完熟期灵武长枣叶为试验材料,通过免疫荧光定位的方法,研究不同发育时期灵武长枣叶阿拉伯半乳糖蛋白(AGPs)的分布特征。结果表明:不同时期叶表皮细胞外切向壁均较厚,抗体所识别的抗原荧光AGPs分布较多,形成了较厚的角质层;垂直于叶片方向的细胞壁较薄,抗体所识别的抗原荧光AGPs分布较少,下表皮气孔保卫细胞分布着少量AGPs。叶肉全部由发达的栅栏组织细胞组成,不同发育时期叶肉细胞壁和细胞内部均密集分布着抗体所识别的抗原荧光AGPs,是叶AGPs分布的主要部位。主脉维管束木质部、形成层和韧皮部细胞壁和细胞内部均分布着大量抗体所识别的抗原荧光AGPs,但快速膨大期主脉AGPs相比其他时期略有减少;而位于叶肉中的侧脉和细脉维管束木质部和韧皮部细胞壁和细胞内部始终分布着大量抗体所识别的抗原荧光AGPs;不同时期主脉、侧脉和细脉维管束鞘中均没有抗体所识别的抗原荧光AGPs分布,可能与维管束鞘细胞中分泌物的形成有关。主脉和侧脉处表皮下的机械组织和薄壁细胞细胞壁分布着较多抗体所识别的抗原荧光AGPs;薄壁组织中的分泌道内部没有AGPs荧光分布。以上结果表明不同时期叶表皮细胞外切向壁AGPs分布较多,形成了较厚的角质层;叶肉始终是叶AGPs分布的主要部位;除快速膨大期叶主脉维管束AGPs略少外,叶维管束内部均分布大量AGPs;叶不仅形成了适应于干旱的结构,而且形成了相应的AGPs分布特征。The early bulking period,the rapid enlargement period,the coloring period and the maturation period leaf of Ziziphus jujuba Mill cv.Lingwuchangzao were used as experimental material to study the distribution of arabinogalactan proteins AGPs using methods of immunofluorescence localization in different development periods.The results showed that the external tangential cell walls of leaf epidermis cells in different developmental stages were all quite thick,antigen fluorescence of AGPs recognized by antibody distributed copiously,and formed fairly thick cuticles.The cell walls perpendicular to leaf blade were quite thin,antigen fluorescence of AGPs recognized by antibody distributed sparsely,stomatal guard cells of lower epidermis distributed a few AGPs.The mesophyll consisted entirely of developed palisade tissue cells,the cell walls and cell interiors of the mesophyll distributed densely antigen fluorescence of AGPs recognized by antibody in different periods leaf,the mesophyll was the main tissue of leaf AGPs distribution.The cell walls and cell interiors of xylem,phloem,and cambium of vascular bundles in main veins distributed mass antigen fluorescence of AGPs recognized by antibody,but AGPs of main veins in the rapid enlargement period decreased slightly compared with other periods.However,the cell walls and cell interiors of xylem and phloem of vascular bundles in lateral veins and veinlets located in the mesophyll distributed all along mass antigen fluorescence of AGPs recognized by antibody.There was no antigen fluorescence of AGPs recognized by antibody in the vascular bundle sheaths of main veins,lateral veins and veinlets,which might be related to the formation of secretions in the vascular bundle sheaths.Mechanical tissues and parenchyma cell walls beneath the epidermis located at main veins and lateral veins distributed much antigen fluorescence of AGPs recognized by antibody,with no distribution in the secretory ducts located at parenchyma.All the results above indicated that there were some differen

关 键 词：灵武长枣 叶片 AGPS 免疫荧光定位 旱生植物 

分 类 号：S665.1[农业科学—果树学] Q942.6[农业科学—园艺学]