Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp.PCC 6803  

作　　者：Tao Sun Shubin Li Guangsheng Pei Lei Chen Weiwen Zhang 

机构地区：[1]Laboratory of Synthetic Microbiology,School of Chemical Engineering&Technology,Tianjin University,Tianjin 300072,China [2]Frontier Science Center for Synthetic Biology&Key Laboratory of Systems Bioengineering,Ministry of Education of China,Tianjin 300072,China [3]Center for Biosafety Research and Strategy,Tianjin University,Tianjin 300072,China

出　　处：《Chinese Journal of Chemical Engineering》2023年第7期128-134,共7页中国化学工程学报（英文版）

基　　金：supported by grants from the National Key Research and Development Program of China(2020YFA0906800,2021YFA0909700,2018YFA0903600 and 2019YFA0904600)。

摘　　要：Synthetic biology efforts have also led to the development of photosynthetic cyanobacteria as"autotrophic cell factories"for biosynthesis of various biofuels directly from CO_(2).However,the low tolerance to toxicity of biofuels has restricted the economic application of cyanobacterial hosts.In this study,RNAseq transcriptomics was employed to reveal stress responses to exogenous n-hexane in Synechocystis sp.PCC 6803.Functional enrichment analysis of the transcriptomic data showed that signal transduction systems were induced significantly.To further identify regulatory genes related to n-hexane tolerance,a library of transcriptional regulators(TRs)deletion mutants was then screened for their roles in nhexane tolerance.The results showed that a knockout mutant of slr0724 that encodes an Hta R suppressor protein was more tolerant to n-hexane than the wild type,indicating the involvement of slr0724 in nhexane tolerance.This study provides the foundation for better understanding the cellular responses to n-hexane in Synechocystis sp.PCC 6803,which could contribute to the further engineering of nhexane tolerance in cyanobacteria.

关 键 词：TRANSCRIPTOME n-Hexane tolerance Metabolomics SYNECHOCYSTIS 

分 类 号：TK6[动力工程及工程热物理—生物能]