卵子超排药物PMSG/hCG对糖尿病小鼠血糖的影响  

Effect of drugs for superovulation PMSG/hCG on blood glucose in diabetes mice

作　　者：王美亚 吴秀利 王靖瑶 薛博文 白璐佳 张迪 WANG Meiya;WU Xiuli;WANG Jingyao;XUE Bowen;BAI Lujia;ZHANG Di(School of Biological and Food Engineering,Fuyang Normal University,Fuyang Anhui 236037,China;School of Medicine,Fuyang Normal University,Fuyang Anhui 236037,China)

机构地区：[1]阜阳师范大学生物与食品工程学院,安徽阜阳236037 [2]阜阳师范大学医学院,安徽阜阳236037

出　　处：《阜阳师范大学学报（自然科学版）》2023年第3期70-76,共7页Journal of Fuyang Normal University:Natural Science

基　　金：安徽省高校自然科学基金重点项目(KJ2021A0656);阜阳师范大学校级重点研究项目(2020PTFY01ZD)。

摘　　要：目前,有关代谢病对人类生殖发育影响的研究日益受到重视。但是,对相关研究中使用到的促性腺激素类超数排卵药物会如何影响动物血糖所知甚少。因此,本研究分析了卵子超排药物孕马血清促性腺激素(pregnant mare’s serum gona-dotrophin,PMSG)与人绒毛膜促性腺激素(human chorionic gonadotropin,hCG)对链脲佐菌素(streptozotocin,STZ)法造模后雌性高糖小鼠血糖浓度变化的影响。结果显示,超排药物对正常小鼠和糖尿病小鼠的血糖浓度不显著影响。但在前糖尿病小鼠中,超排药物可以使其发生血糖浓度升高的比率达到77.78%,平均浓度升高4.41 mmol/L。本研究表明,在使用糖尿病雌性动物开展的研究中,需要考虑促性腺激素类超排药物对小鼠血糖水平造成的影响,以及可能对实验体系造成的干扰。这一结果也提示进一步研究促性腺激素在糖尿病发生过程中的角色和机理的重要性。Studies on the effects of metabolic diseases on human reproduction and development have received increasing attention.However,little is known about how gonadotropin-based superovulatory drugs used in related studies affect blood glucose concentrations in animals.Therefore,this study analyzed the effect of the ovarian superovulation drug pregnant mare's serum gonadotropin(PMSG)and human chorionic gonadotropin(hCG)on changes in blood glucose concentrations in female hyperglycemic mice after streptozotocin(STZ)method of modeling.The results show that neither normal nor diabetic mice experienced significant elevated blood glucose concentrations from the supernatant.This study suggests that the effect of gonadotropinbased superdischarge drugs on blood glucose levels in mice needs to be considered in studies using diabetic females,as well as the possible interference with the experimental system.This result also suggests the importance of further research on the role and mechanism of gonadotropins in the development of diabetes mellitus.

关 键 词：超数排卵 糖尿病 血糖 

分 类 号：Q291[生物学—细胞生物学]