时间与功率对BL21大肠杆菌超声破碎效果的影响  

Studies on the Effect of Time and Power on Ultrasonic Fragmentation of BL21 E.Coli

作　　者：鄢树枫 王钰坪 吴珊涛 王晓媛 戴心如 YAN Shufeng;WANG Yuping;WU Shantao;WANG Xiaoyuan;DAI Xinru(School of Resources and Chemical Engineering,Sanming University,Sanming 365004,China)

机构地区：[1]三明学院资源与化工学院,福建三明365004

出　　处：《三明学院学报》2023年第3期77-82,共6页Journal of Sanming University

基　　金：福建省自然科学基金项目(2020J05092);福建省中青年教师教育科研项目(JAT190705);三明学院国家基金培育项目(PYT2004);大学生创新创业训练计划项目(CB220303)。

摘　　要：以BL21大肠杆菌为实验宿主菌,研究时间与功率对BL21大肠杆菌超声破碎效果的影响,设置不同时间和功率梯度对大肠杆菌BL21进行超声破碎,并获取其胞内蛋白质,通过蛋白质浓度检测及考马斯亮蓝(Bradford法)测定蛋白质含量,进而评价大肠杆菌BL21的超声破碎效果。研究结果表明,菌液体积100 mL,菌液浓度为3.772×10^(9) cfu/mL(OD600_(nm)=1.886)时,超声功率对大肠杆菌BL21破碎效果有明显影响;破碎功率固定为5%时,破碎效果与破碎时间呈正比;破碎时间固定为1 min时,破碎功率在1%、3%和9%时其超声破碎效果逐渐提升,证明破碎效果与破碎功率呈线性关系。In this study,BL21 E.coli was used as the experimental host strain to study the effect of time and power on its ultrasonic fragmentation.BL21 E.coli was ultrasonically disrupted at different time and power gradients,and its intracellular proteins were obtained.The protein content was measured by protein concentration detection and Bradford method,and then the ultrasonic fragmentation effect of BL21 E.coli was evaluated.The results showed that the volume of bacterial solution was 100 ml and the concentration of bacterial solution was 3.772×10^(9) cfu/ml(OD600_(nm)=1.886),the ultrasonic power had a significant effect on the crushing effect of BL21 E.coli.When the crushing power is fixed at 5%,the crushing effect is proportional to the crushing time.When the crushing time is fixed at 1 min,the ultrasonic crushing effect is gradually improved when the crushing power is 1%,3%and 9%,which proves that the crushing effect is linearly related to the crushing power.The results of this study provide reference value for the research and application of ultrasonic disruption of E.coli.

关 键 词：BL21大肠杆菌 超声破碎 功率 时间 蛋白质浓度 

分 类 号：Q93-33[生物学—微生物学]