31份小麦材料中抗旱基因的KASP检测  

Analysis of Drought Resistant Genes in 31 Wheat Breeding Materials by KASP Marker

作　　者：李玮[1] 孔淑鑫 宋国琦[1] 李玉莲[1] 张淑娟[1] 张荣志[1] 高洁[1] 李吉虎 樊庆琦[1] 李根英[1] LI Wei;KONG Shuxin;SONG Guoqi;LI Yulian;ZHANG Shujuan;ZHANG Rongzhi;GAO Jie;LI Jihu;FAN Qingqi;LI Genying(Crop Research Institute,Shandong Academy of Agricultural Sciences/National Engineering Research Center of Wheat and Maize/Key Laboratory of Wheat Biology and Genetics and Breeding in Northern Huang-huai River Plain,Ministry of Agriculture and Rural Affairs/Shandong Technology Innovation Center of Wheat,Jinan,Shandong 250100,China;College of Agricultural Science and Technology,Shandong Agriculture and Engineering University,Dezhou,Shandong 251100,China)

机构地区：[1]山东省农业科学院作物研究所/小麦玉米国家工程研究中心/农业农村部黄淮北部小麦生物学与遗传育种重点实验室/山东省小麦技术创新中心,山东济南250100 [2]山东农业工程学院农业科技学院,山东德州251100

出　　处：《麦类作物学报》2023年第10期1241-1247,共7页Journal of Triticeae Crops

基　　金：山东省农业科学院农业科技创新工程项目(CXGC2022A01);山东省良种工程项目(2021LZGC009)。

摘　　要：为明确小麦材料中抗旱相关基因的组成,利用已报道的29个标记(11个新转化的KASP标记以及已报道的18个KASP标记),对16份抗旱小麦品种和15份高产小麦品种进行KASP检测。结果表明,多数基因位点在抗旱小麦品种和高产小麦品种间的优异等位基因数目无明显差别;TaPYL1-1B、Wcor14-2B、TaSRL1-4A、TaSnRK2.3-1A、QTDW.caas-6BL位点的优异等位基因在旱地品种中占比较高,而TaWRKY51-2B和TaSnRK2.4-3A位点的优异等位基因仅存在于抗旱品种中,前者的供体材料为晋麦47、晋麦92和晋麦101,后者的供体材料为济麦379。To clarify drought resistant alleles in wheat materials,16 drought resistant wheat cultivars and 15 high-yield wheat cultivars were detected by 29 KASP markers(11 newly converted KASP markers and 18 reported KASP markers)related to drought resistance.The result showed that there was no significant difference in the number of prior elleles between drought resistant cultivars and high-yield cultivars for most of the gene loci.The percentage of prior alleles of TaPYL1-1B ,Wcor14-2B ,TaSRL1-4A ,TaSnRK2.3-1A and QTDW.caas-6BL loci were higher in drought resistant cultivars than in high-yield cultivars.The prior alleles of TaWRKY51-2B and TaSnRK2.4-3A loci were only detected in drought resistant cultivars,and the former donor materials were Jinmai 47,Jinmai 92 and Jinmai 101,and the latter donor material was Jimai 379.

关 键 词：小麦 抗旱 分子标记 育种亲本 

分 类 号：S512.1[农业科学—作物学] S330