SDS-PAGE电泳蛋白染色方法研究进展  

Research Progress of SDS-PAGE Electrophoretic Protein Staining Method

作　　者：迟玲玲 王京燕 于娜 盖维娜 申欣宜 Chi Lingling;Wang Jingyan;Yu Na;Gai Weina;Shen Xinyi(Shandong Junxiu Biotechnology Co.,Ltd.,Yantai 264006,China)

机构地区：[1]山东隽秀生物科技股份有限公司,山东烟台264006

出　　处：《山东化工》2023年第15期117-119,共3页Shandong Chemical Industry

摘　　要：SDS-PAGE电泳结束后,蛋白质条带染色是其中一个重要的步骤。目前蛋白质染色方法主要有考马斯亮蓝染色法、银染法、荧光染色法、负染法等,其中考马斯亮蓝染色法又分为传统考染和胶体考染;银染又分为银染A法和银染B法;每种方法各有利弊,目前,对染色方法改进主要集中在如何提高蛋白质染色的灵敏度,缩短染色时间,用无毒的化学物质去替换现在凝胶电泳中常用的有毒物等方面。如何在保持方法高灵敏度的同时,又能够有效地降低染色背景是亟待解决的问题,也是未来的发展方向。After the completion of SDS-PAGE electrophoresis,protein band staining is one of the important steps.At present,the protein staining methods mainly include Coomassie bright blue staining method,silver staining method,fluorescent staining method,negative staining method,etc.,among which Coomassie bright blue staining method is divided into traditional test dyeing and colloidal staining.Silver dyeing is further divided into silver dyeing A method and silver dyeing B method.Each method has its own advantages and disadvantages,and at present,the improvement of staining methods mainly focuses on how to improve the sensitivity of protein staining,shorten the staining time,and replace the toxic substances commonly used in gel electrophoresis with non-toxic chemicals.How to maintain the high sensitivity of the method while effectively reducing the staining background is an urgent problem to be solved,and it is also the future development direction.

关 键 词：蛋白染色方法 考马斯亮蓝 蛋白质条带 

分 类 号：TQ617[化学工程—精细化工]