基于聚(N-异丙基丙烯酰胺)的热响应性细胞培养表面及其在细胞片工程的应用研究进展  

Research Progress of Poly(N-isopropylacrylamide)-based Thermoresponsive Cell Culture Surface and Its Application in Cell Sheet Engineering

作　　者：章彧 谢采云 王铭博 蓝翔 李玲玲 吴丽 Zhang Yu;Xie Caiyun;Wang Mingbo;Lan Xiang;Li Lingling;Wu Li(School of Medical,Wuhan College of Arts&Sciences,Wuhan 430345,China)

机构地区：[1]武汉文理学院医学院,湖北武汉430345

出　　处：《山东化工》2023年第15期122-126,137,共6页Shandong Chemical Industry

基　　金：湖北省教育厅科学技术研究计划指导性项目(B2022479);武汉文理学院2022年校级教(科)研立项项目(2022xk08)。

摘　　要：通过接枝聚N-异丙基丙烯酰胺(PIPAAm)制备的热响应细胞培养表面,在外部温度变化后显示受控的细胞黏附和分离特性,可用于制造细胞片。对基于热响应性细胞培养表面的细胞片制备、细胞片操作和移植的广泛研究建立了细胞片工程的跨学科领域,使其成为组织工程和再生医学中一项有前途和有吸引力的技术。本综述整理各种类型的PIPAAm基热响应表面的制备方法、性质,并介绍了细胞片的制备及细胞片工程用于治疗和再生受损人体组织的研究进展。Thermoresponsive cell culture surface prepared by grafting poly(N-isopropylacrylamide)(PIPAAm)or its copolymer,shows controlled cell adhesion and detachment following external temperature changes,and can be used to manufacture cell sheets.Extensive research on the preparation,operation and transplantation of cell sheets based on thermoresponsive surface,has established an interdisciplinary field of cell sheet engineering,making it a promising and attractive technology in tissue engineering and regenerative medicine.This review summarizes the preparation methods and properties of various types of PIPAAm modified thermoresponsive surfaces,and introduces the research progress of cell sheet preparation and cell sheet engineering for the treatment and regeneration of damaged human tissues.

关 键 词：聚(N-异丙基丙烯酰胺) 热响应 细胞片 移植 再生医学 

分 类 号：TQ317[化学工程—高聚物工业]