HPLC法同时测定蒲苓盆炎康颗粒中4种成分的含量  

Simultaneous Determination of Four Components in Pulingpenyankang Granules by HPLC

作　　者：李志娇 于靖 Li Zhijiao;Yu Jing(Linyi Inspection and Testing Center,Linyi 276001,China;Department of Reproductive Medicine,Linyi Maternal and Child Health Hospital,Linyi 276000,China)

机构地区：[1]临沂市检验检测中心,山东临沂276001 [2]临沂市妇幼保健院生殖医学科,山东临沂276000

出　　处：《山东化工》2023年第15期150-152,155,共4页Shandong Chemical Industry

摘　　要：目的:建立高效液相色谱同时测定蒲苓盆炎康颗粒中丹参素钠、丹酚酸B、迷迭香酸、绿原酸的含量。方法:以Agilent ZORBAX SB-C 18柱(4.6 mm×250 mm,5μm)为色谱柱,选用乙腈(A)-1%醋酸(B)为流动相梯度洗脱;检测波长为286 nm、流速为1.0 mL/min,柱温为25℃。结果:丹参素钠进样量在0.0233~0.2798μg(r=0.9995)、丹酚酸B进样量在0.0203~0.2026μg(r=0.9998)、迷迭香酸进样量在0.0061~0.0737μg(r=0.9998)、绿原酸进样量在0.0123~0.1479μg(r=0.9997)范围内有较好的线性关系;丹参素钠平均回收率为98.5%,丹酚酸B平均回收率为97.8%,迷迭香酸平均回收率为98.2%,绿原酸平均回收率为97.9%,RSD均在2.0%以内。结论:本测定方法简便,准确灵敏、重复性较好,为蒲苓盆炎康颗粒在临床应用提供理论依据。Objective:To establish a HPLC method for the simultaneous determination of danshensu sodium,salvianolic acid B,rosmarinic acid and chlorogenic acid in Pulingpenyankang granules.Methods:Agilent ZORBAX SB-C 18 column(4.6 mm×250 mm,5μm)is the chromatographic column,and acetonitrile(A)-1%acetic acid(B)is selected as the mobile phase for gradient elution.The detection wavelength is 286 nm,the flow rate is 1.0 mL/min,and the column temperature is 25℃.Results:The injection volume of danshensu sodium is 0.0233~0.2798μg(r=0.9995),salvianolic acid B injection volume is 0.0203~0.2026μg(r=0.9998),rosmarinic acid injection volume is 0.0061~0.0737μg(r=0.9998),chlorogenic acid injection volume is 0.0123~0.1479μg(r=0.9997)with good linear relationship in the range.The average recoveries of salvianolic acid B,rosmarinic acid and chlorogenic acid were 98.5%,97.8%,98.2%and 97.9%respectively,and the RSD was within 2.0%.Conclusion:This method is simple,accurate,sensitive and reproducible,which provides a theoretical basis for the clinical application of Pulingpenyankang granules.

关 键 词：蒲苓盆炎康颗粒 丹参素钠 丹酚酸B 迷迭香酸 绿原酸 高效液相色谱法 

分 类 号：O657.7[理学—分析化学] R927.2[理学—化学]