马尾松树高生长性状SSR标记关联验证  

Verification of SSR Markers Significantly Associated with Height Growth Traits in Pinus massoniana

作　　者：凌金燕 冯源恒[2,3] 杨章旗[2,3] 陈虎[2,3] 谭健晖[2,3] 龚桂芳[2,3] 罗群凤 付军 翟章贵 LING Jinyan;FENG Yuanheng;YANG Zhangqi;CHEN Hu;TAN Jianhui;GONG Guifang;LUO Qunfeng;FU Jun;ZHAI Zhanggui(College of Life Science,Guangxi Normal University,Guilin Guangxi 541006,China;Guangxi Academy of Forestry,Nanning Guangxi 530002,China;Guangxi Masson Pine Engineering Technology Research Center/Key Laboratory of Mid-South Fast-growing Timber Breeding of State Forestry and Grassland Administration/Guangxi Key Laboratory of Cultivation of Excellent Timber Resources/Masson Pine Engineering Technology Research Center of State Forestry and Grassland Administration(Guangxi Academy of Forestry),Nanning Guangxi 530002,China;Guangxi Paiyangshan Forest Farm,Ningming Guangxi 532500,China)

机构地区：[1]广西师范大学生命科学院,广西桂林541006 [2]广西壮族自治区林业科学研究院,广西南宁530002 [3]广西马尾松工程技术研究中心/国家林业和草原局中南速生材繁育重点实验室/广西优良用材林资源培育重点实验室/国家林业和草原局马尾松工程技术研究中心(广西壮族自治区林业科学研究院),广西南宁530002 [4]广西国有派阳山林场,广西宁明532500

出　　处：《广西师范大学学报（自然科学版）》2023年第5期152-160,共9页Journal of Guangxi Normal University:Natural Science Edition

基　　金：八桂学者专项(2019A26);广西科技基地和人才专项(桂科AD19254004)。

摘　　要：分子标记辅助选择育种是获得控制目标性状基因的重要方法。本研究以马尾松二代全同胞家系为材料,选择4个课题组前期获得的与马尾松生长性状关联的SSR标记:PCZ002、PCZ023、PCZ142和PCZ187,每个标记选择4个杂交家系,分别有150、172、157、163份单株材料,结合树高生长性状数据对马尾松二代全同胞群体进行关联分析。结果表明,标记PCZ142扩增出的PCZ142-AA和PCZ142-AB基因型与树高存在正相关性,且树高均显著高于PCZ142-BB基因型材料,而PCZ142-BB基因型与树高存在显著负相关(P<0.05);标记PCZ187扩增出的PCZ187-BB基因型与树高存在极显著正相关性(P<0.01),且树高显著高于PCZ187-AA和PCZ187-AB基因型材料;标记PCZ002和PCZ023的各基因型与树高无显著相关性。综上,标记PCZ142-BB和PCZ187-BB与马尾松树高生长性状存在显著相关性,PCZ142-BB为显著负相关,PCZ187-BB为极显著正相关,这2种基因型材料可成为马尾松树高生长性状重要的基因资源。本研究结果为定位控制群体中马尾松树高生长性状位点提供重要信息,并为马尾松分子标记辅助选择育种提供参考。Molecular marker-assisted selection breeding is an important method to obtain genes controlling target traits.In this study,four SSR markers(PCZ002,PCZ023,PCZ142 and PCZ187)associated with the growth traits of P.massoniana were selected from the second-generation full-sib families of P.massoniana.Four hybrid families were selected for each marker,with 150,172,157 and 163 individual materials,respectively.The association analysis of the second-generation full-sib population of P.massoniana was carried out by combining the data of tree height growth traits.The results showed that the genotypes of PCZ142-AA and PCZ142-AB were positively correlated with tree height,and the tree height was significantly higher than that of PCZ142-BB genotype,while PCZ142-BB genotype was significantly negatively correlated with tree height(P<0.05).There was a significant positive correlation between PCZ187-BB genotype and tree height(P<0.01),and the tree height was significantly higher than that of PCZ187-AA and PCZ187-AB genotype materials.The genotypes marked PCZ002 and PCZ023 had no significant correlation with tree height.In summary,the markers PCZ142-BB and PCZ187-BB were significantly correlated with the height growth traits of P.massoniana.The PCZ142-BB was significantly negatively correlated,and the PCZ187-BB was significantly positively correlated.These two genotype materials could become important genetic resources for the height growth traits of P.massoniana.The results of this study provide important information for the locus of height growth traits of P.massoniana in the positioning control population,and provide reference for molecular marker-assisted selection breeding of P.massoniana.

关 键 词：马尾松 SSR标记 树高 关联分析 辅助育种 

分 类 号：S791.248[农业科学—林木遗传育种] Q7[农业科学—林学]