一株海洋真菌与细菌共培养次级代谢产物的研究  

Research on secondary metabolites of a marine-derived fungus co-cultured with a bacteria

作　　者：朱夏濠 郝宝聪 郑杨 李申奥 徐奥翔 王梦如 陈敏 ZHU Xiahao;HAO Baocong;ZHENG Yang;LI Shen'ao;XU Aoxiang;WANG Mengru;CHEN Min(College of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127,China)

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127

出　　处：《扬州大学学报（农业与生命科学版）》2023年第4期84-89,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(81703411)。

摘　　要：对海洋真菌Penicillium janthinellum HK1-6与耐甲氧西林金黄色葡萄球菌(Staphylococcus aureus)ATCC 43300共培养的次级代谢产物进行分离纯化与结构鉴定,综合运用多种色谱方法从共培养发酵产物中分离得到5个化合物,采用NMR等光谱方法并参照文献将其分别鉴定为吲哚-3-乙酸(1)、青霉酸(2)、diorcinol(3)、orcinol(4)和paraherquanide N(5)。同时采用质谱-分子网络及GNPS平台技术鉴定了3个吲哚生物碱类化合物notoamide B(6)、paraherquanide F(7)和paraherquamide E(8)。该研究表明,共培养是激活微生物沉默基因簇,发现抗生素类化合物的一种有效方法。Five secondary metabolites were isolated from the co-culture fermentation products of the marine-derived fungus Penicillium janthinellum HK1-6 and the methicillin-resistant Staphylococcus aureus ATCC 43300 by comprehensive use of chromatographic methods.These compounds were determined as indole-3-acetic acid(1),penicillic acid(2),diorcinol(3),orcinol(4),and paraherquanide N(5)by detailed analysis of the NMR spectroscopic data and by comparison with data reported in the literatures.Three indole alkaloids,including notoamide B(6),paraherquanides F(7),and E(8),were identified by using mass spectrometry-molecular network and GNPS platform techniques.This study indicates that co-culture is an effective method for activating microbial silencing gene clusters and discovering antibiotic compounds.

关 键 词：海洋真菌 细菌 共培养 次级代谢产物 分子网络 吲哚生物碱 

分 类 号：S154.39[农业科学—土壤学] R378.11[农业科学—农业基础科学]