不同矿化水对纳米纤维素-铁螯合物的影响及其在矫治梨缺铁黄化中的应用  

Effects of mineralized water on nanocellulose-iron chelates formulation and the application on plant iron deficiency chlorosis remediation

作　　者：边艺伟 郭献平[2] 王东升[2] 姚春玲 毋青男 赵凡 吴中营[2] 何睿[3] 王合中 BIAN Yiwei;GUO Xianping;WANG Dongsheng;YAO Chunling;WU Qingnan;ZHAO Fan;WU Zhongying;HE Rui;WANG Hezhong(Yanling County Ganluo Health Care Company Limited,Xuchang 461200,China;Institute of Horticulture,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]鄢陵县甘罗养生养老有限公司,河南许昌461200 [2]河南省农业科学院园艺研究所,郑州450002 [3]河南农业大学植物保护学院,郑州450002

出　　处：《扬州大学学报（农业与生命科学版）》2023年第4期119-127,共9页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：河南省重点研发与推广专项(科技攻关项目)(202102110412);河南省重大科技专项(221100110400);河南省农业科学院科技创新团队项目(2023TD27);河南省中央引导地方科技发展专项(YDZX20214100003354);国家现代农业产业技术体系专项(CARS-28)。

摘　　要：采用不同矿化度水质配制纳米纤维素(NC)-铁(Fe)螯合物(NC-Fe),研究不同矿化度水对NC-Fe的螯合及其对梨树缺铁黄化矫治的影响。结果表明:用矿化度为750、1500、3000 mg·L^(-1)的矿化水配制的NC-Fe悬浮液,其纳米粒子粒径、Zeta电位值随水矿化度的增加而变大;随水矿化度的升高,纳米螯合物的生物活性降低,与NC-Fe处理相比,由750、1500、3000 mg·L^(-1)矿化水配制的NC-Fe处理叶片活性铁含量分别下降9.1%、23.6、40.3%。此外,与NC-Fe处理相比,随水矿化度的升高,其配制的NC-Fe处理叶片内铁蛋白基因Fer1的相对表达量显著下降。综上,当水矿化度小于1500 mg·L^(-1)时,矿化度对NC-Fe的螯合有限,但仍能促进叶片有效铁含量提升,对铁蛋白基因Fer1的表达有诱导作用,故可在生产中使用Ⅲ类以下的矿化水配制NC-Fe,用于矫治梨树缺铁黄化。This research studied the effects of different salinity levels of water on NC-Fe formulation and its application in remediation of iron deficiency chlorosis(IDC).The results showed that the particle size and Zeta potential of NC-Fe chelates were significantly increased with the increase of concentrations of mineralization level of water.Compared with NC-Fe formulated in 750 mg·L^(-1) of mineralization water(NC-Fe-750),the particle size and Zeta potential of NC-Fe chelated within 1500(NC-Fe-1500),3000 mg·L^(-1)(NC-Fe-3000)of mineralized water were increased.Compared with the treatment of NC-Fe formulated in DI water,the active iron in IDC leaves treated with NC-Fe-750,NC-Fe-1500 and NC-Fe-3000 decreased by 9.1%,23.6% and 40.3%,respectively.Moreover,compared with NC-Fe-DI,the relative expression of ferritin gene Fer1 in IDC leaves was decreased significantly with the increase of water mineralization in NC-Fe formulation.When the mineralization of water was less than 1500 mg·L^(-1),the salinity of water had limited chelation effect on NC-Fe,but it could promote the content of active iron in leaves and induce the expression of ferritin gene Fer1.Therefore,NC-Fe can be prepared with the mineralization of water below class Ⅲ to remediate IDC of pear trees.

关 键 词：梨 缺铁黄化矫治 纳米纤维素-铁螯合剂 活性铁 铁蛋白基因 

分 类 号：S661.2[农业科学—果树学]