涡旋辅助-可切换型溶剂液相微萃取用于中药样品中肉桂酸衍生物的分析  

Vortex-assisted switchable solvent liquid-phase microextraction for preconcentration of cinnamic acid derivatives in traditional Chinese medicine

作　　者：范博[1] 杨笑 胡爽[1] Bo Fan;Xiao Yang;Shuang Hu(School of Pharmacy,Shanxi Medical University,Taiyuan 030001,Shanxi,China)

机构地区：[1]山西医科大学药学院,山西太原030001

出　　处：《Journal of Chinese Pharmaceutical Sciences》2023年第7期551-559,共9页中国药学（英文版）

基　　金：The National Natural Science Foundation China(Grant No.81973287)。

摘　　要：本文以可切换型溶剂己酸取代毒性较大的有机溶剂作为萃取溶剂、一次性注射器作为萃取装置,借助涡旋搅拌的萃取动力,建立了涡旋辅助-可切换型溶剂液相微萃取(VA-SSLPME),并结合HPLC对中药样品中4种肉桂酸类衍生物(咖啡酸、对羟基肉桂酸、阿魏酸和芥子酸)进行了富集和测定。本文对影响4种目标化合物富集倍数的关键因素进行了优化,在最优条件下进行方法学考察,并对萃取机理进行了分析和阐释。结果表明,VA-SSLPME对目标分析物的富集倍数在7–57之间,准确度和精密度符合要求,检测限和定量限分别为2.0–7.0和5.0–15 ng/mL。本研究建立了一种简单、快速、环境友好型的新型微萃取方法,并结合HPLC成功用于中药样品中微量肉桂酸类衍生物的提取、富集和测定。In the present study,we developed a vortex-assisted switchable solvent liquid-phase microextraction(VA-SSLPME)approach to extract four cinnamic acid derivatives in traditional Chinese medicinal samples prior to HPLC analysis.During the extraction process,hypotoxic hexanoic acid was used as a switchable polarity extraction solvent.First,hydrophobic hexanoic acid was converted into a hydrophilic form by the addition of sodium carbonate.Then,sulfuric acid was added to lower the pH value of the mixture,which induced the retransition of hexanoate ion into hexanoic acid,generating carbon dioxide(CO_(2))bubbles in situ.The formation of CO_(2) bubbles further facilitated the rise of hexanoic acid and expedited the separation of the organic phase from the aqueous phase.Herein,the critical parameters that affect the enrichment factor were optimized,including the type and amount of extractant,the volume of sodium carbonate solution,ionic strength,mineral acid type,and extraction time.Under the optimized conditions,enrichment factors of target analytes were 7–57 with satisfactory accuracies and precision.The limits of detection and quantitation were 2.0–7.0 and 5.0–15 ng/mL,respectively.In the present work,we successfully applied the proposed method for the simultaneous extraction,enrichment,and determination of four cinnamic acid derivatives in traditional Chinese medicinal samples.

关 键 词：可切换型溶剂 涡旋辅助液相微萃取 中药样品 肉桂酸类衍生物 

分 类 号：R917[医药卫生—药物分析学]