贻贝启发的抗菌骨粘合剂的制备与性能  

Preparation and Properties of Mussel-Inspired Antibacterial Bone Adhesive

作　　者：刘嘉辉 安佰超 闫秋艳 栾世方[1,2] LIU Jia-Hui;AN Bai-Chao;YAN Qu-Yan;LUAN Shi-Fang(State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China;School of Pharmacy,Guangdong Medical University,Dongguan 523000,China;School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510000,China)

机构地区：[1]中国科学院长春应用化学研究所,高分子物理与化学国家重点实验室,长春130022 [2]中国科学技术大学应用化学与工程学院,合肥230026 [3]广东医科大学药学院,东莞523000 [4]南方医科大学中医药学院,广州510000

出　　处：《应用化学》2023年第9期1258-1266,共9页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金青年基金(No.22107099);国家重点研发计划(No.2021YFC2101700);广东省自然科学基金(No.2018A030313355);中国科学院威高发展计划资助。

摘　　要：骨粘合剂因其快速且稳定的骨粘结性能而受到广泛关注。然而,商用粘合剂的较差生物相容性所导致的不良反应限制了其广泛使用。基于贻贝粘附剂的启发,利用氧化透明质酸(OHA)、单宁酸(TA)与聚赖氨酸(PL)三者共混制备了一种生物相容性良好的双交联网络抗菌骨粘合剂。该粘合剂的粘合粘结强度~450 kPa,搭接粘结强度为~250 kPa,其优异的粘附性能,自愈合性与可注射性可以满足多种临床应用场景,其良好的体外生物相容性为骨细胞增殖和组织愈合提供了稳定的生长环境。此外,单宁酸的抗菌性能使该粘合剂可杀死95%以上的金黄葡萄球菌与大肠杆菌,降低了骨折处发生细菌感染的风险。As one of the most commonly occurred trauma conditions,bone fractures have harmed the health of tens of millions of people.Bone adhesives have attracted wide attention due to the excellent adhesive property,however,poor biocompatibility may become a barrier hindering fracture healing,which restricts the wide use of bone adhesives.Inspired by mussel,a novel antibacterial bone adhesive is fabricated by blending the oxidized hyaluronic acid with tannic(TA)and poly-ε-lysine(PL).Due to the electrostatic interaction and dynamic covalent bond,the bone adhesive exhibits prominent adhesive,self-healing and injectable prosperities,which could meet the different needs of clinical applications.The great cytocompatibility of the adhesive could offer an environment suitable for bone tissue healing.Moreover,the antibacterial activity of the adhesive that provided by TA could decrease the risk of bacterial infection at the site of fracture.

关 键 词：双交联网络骨粘合剂 贻贝仿生 可注射性 生物相容性 抗菌性 

分 类 号：O636[理学—高分子化学]