基于温敏性N,N-二甲基丙烯酰胺/N-异丙基丙烯酰胺无规共聚物的毛细管无胶电泳筛分介质  

Thermoresponsive Poly(N,N-dimethylacrylamide-co-N-isopropylacrylamide)for Preparation of Composite Sieving Media in Non-Gel Capillary Electrophoresis

作　　者：江凤浩 洪瀚 姜伯玮[4] 苏朝晖[1,2] JIANG Feng-Hao;HONG Han;JIANG Bo-Wei;SU Zhao-Hui(State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;University of Science and Technology of China,Hefei 230026,China;Lifepolymer Composite Material Technology Co.,Ltd.,Taizhou 225321,China;The First Research Institute of the Ministry of Public Security,Beijing 100048,China)

机构地区：[1]中国科学院长春应用化学研究所,高分子物理与化学国家重点实验室,长春130022 [2]中国科学技术大学,合肥230026 [3]莱宝利复合材料科技有限公司,泰州225321 [4]公安部第一研究所,北京100048

出　　处：《应用化学》2023年第9期1312-1321,共10页Chinese Journal of Applied Chemistry

摘　　要：本文合成了N,N-二甲基丙烯酰胺/N-异丙基丙烯酰胺无规共聚物(P(DMA-co-NIPAM))和聚N,N-二甲基丙烯酰胺(PDMA),并将二者共混制备毛细管无胶电泳筛分介质,旨在降低筛分介质粘度的同时增强其对DNA的分离性能。核磁共振氢谱(1H NMR)和傅里叶变换红外光谱(FT-IR)证明了2种聚合物的成功合成。表征了P(DMA-co-NIPAM)/PDMA共混物溶液的流体力学直径(Dh)、低剪切粘度和吸光度,结果表明,P(DMAco-NIPAM)聚合物具有温敏性,低临界溶解温度(LCST)为60~80℃。DNA筛分结果显示,PDMA中添加了10%P(DMA-co-NIPAM)的复合筛分介质性能最好,相比PDMA粘度降低19%以上,对大DNA片段的分辨率和理论塔板数均明显提高,分离时间缩短4%,显示了优良的分离性能。In this paper,a random copolymer of N,N-dimethylacrylamide and N-isopropylacrylamide(P(DMA-co-NIPAM))as well as poly-N?,?N-dimethylacrylamide(PDMA)are synthesized and blended to prepare a composite sieving medium for non-gel capillary electrophoresis,aiming to reduce the viscosity of the sieving medium while enhance its DNA separation performance.Analyses by NMR spectroscopy and Fourier transform infrared(FT-IR)spectroscopy confirm that both polymers are successfully synthesized.The hydrodynamic diameter,low shear viscosity and UV-Vis absorbance results show that P(DMA-co-NIPAM)is thermoresponsive with a low critical solution temperature(LCST)of 60~80℃.DNA sieving results indicate that the composite medium containing 10%P(DMA-co-NIPAM)exhibits best properties,a viscosity 19%lower than PDMA and a higher resolution and theoretical plate number in separating large DNA fragments,and separation time is reduced by more than 4%,showing good separation performance.

关 键 词：毛细管电泳 毛细管无胶电泳筛分介质 DNA分离 温敏性聚合物 聚N N-二甲基丙烯酰胺 

分 类 号：O631[理学—高分子化学]