电化学厌氧消化代谢途径中关键节点处酶活性的研究  

Study on enzyme activity at key nodes in EAD metabolic pathway

作　　者：刘海波 刘洪周 王楠 陈铁柱 李建昌[1] LIU Haibo;LIU Hongzhou;WANG Nan;CHEN Tiezhu;LI Jianchang(School of Energy And Environmental Sciences,Yunnan Normal University,Kunming,650500,China)

机构地区：[1]云南师范大学能源与环境科学学院,昆明650500

出　　处：《环境化学》2023年第9期3179-3187,共9页Environmental Chemistry

基　　金：国家自然科学基金(21968038)资助.

摘　　要：本研究以活性污泥作为接种物,葡萄糖作为底物,电解电压作为扰动因素,采用通量平衡分析(FBA)的方法,构建电化学厌氧消化(EAD)的代谢网络,并对其通量平衡分析和关键代谢节点的信息进行研究.此外,针对特定关键节点的通量变化情况,结合EAD体系中关键酶活性变化进行分析.实验结果表明,通过对EAD体系的FBA和关键节点进行研究,发现EAD体系中CH_(4)的合成途径是以乙酸转化途径占主导地位,其次为H_(2)还原CO_(2)途径,EAD体系的这两条途径的占CH_(4)产生总量的64.95%和35.05%.在关键酶活性检测中,关键酶的活性与产甲烷途径显示出较好的一致性.在0.6 V扰动下,关键酶活性都有所增加,催化微生物体内反应加快,与代谢通量变化有较高的相关性.研究还发现在电压扰动下EAD体系中H_(2)还原CO_(2)产生的产甲烷通量13.44%和12.76%均高于对照组5.97%,进而提升了EAD系统的整体产甲烷能力.In this study,activated sludge was used as the inoculum,glucose was used as the substrate,and the electrolytic voltage was used as the disturbance factor.The method of flux balance analysis(FBA)was used to construct the metabolic network of EAD,and its flux balance analysis(FBA)and key Metabolic node information for research.In addition,the flux changes of specific key nodes were analyzed in combination with the changes of key enzyme activities in the EAD system.The experimental results show that:by studying the FBA and key nodes of the EAD system,it is found that the synthesis pathway of CH_(4)in the EAD system is dominated by the acetate conversion pathway,followed by the H_(2)reduction CO_(2)pathway,and the two pathways in the EAD system account for CH_(4).Generated 64.95%and 35.05%of the total.In the detection of key enzyme activities,the activities of key enzymes showed good consistency with the methanogenesis pathway.Under 0.6 V perturbation,the activities of key enzymes increased,and the reactions in the catalyzed microorganisms accelerated,which had a high correlation with the changes in metabolic flux.The study also found that under voltage disturbance,the methane production fluxes of H_(2)reduction CO_(2)in the EAD system were 13.44%and 12.76%higher than those in the control group(5.97%),thereby improving the overall methane production capacity of the EAD system.

关 键 词：电化学厌氧消化(EAD) 代谢通量分析 酶活性 

分 类 号：X703[环境科学与工程—环境工程]