四川省泸州市一起流行性脑膜炎疫情病原分离株分子特征及耐药性分析  

Molecular characteristics and resistance of pathogen strains from a case of epidemic meningitis epidemic in Luzhou,Sichuan

作　　者：刘小红 李丹 毛亚 姜敏 许文平[1] 邹家凤[1] 蔡金桦 曾林子[2] 余光才 Liu Xiaohong;Li Dan;Mao Ya;Jiang Min;Xu Wenping;Zou Jiafeng;Cai Jinhua;Zeng Linzi;Yu Guangcai(Luzhou Prefectural Center for Disease Control and Prevention,Luzhou 646200,Sichuan,China;Sichuan Provincial Center for Disease Control and Prevention,Chendu 610000,Sichuan,China;Jiangyang District Center for Disease Control and Prevention,Luzhou 646000,Sichuan,China)

机构地区：[1]泸州市疾病预防控制中心,四川泸州646300 [2]四川省疾病预防控制中心,四川成都610000 [3]泸州市江阳区疾病预防控制中心,四川泸州646000

出　　处：《疾病监测》2023年第8期977-982,共6页Disease Surveillance

基　　金：四川省科技厅项目(No.2022ZDZX0017)。

摘　　要：目的分析2022年四川省泸州市一起流行性脑脊髓炎疫情的病原种类及分子特征。方法实时荧光定量反转录聚合酶链式反应(RT-PCR)检测病例中脑膜炎奈瑟菌基因片段,巢式PCR扩增管家基因并测序。对密切接触者分离出的脑膜炎奈瑟菌进行血清分群、脉冲场凝胶电泳(PFGE)分子分型、药敏试验和基因测序,分析菌株遗传进化关系。结果实时荧光定量RTPCR结果显示病例为W群脑膜炎奈瑟菌感染,巢式PCR扩增出3条管家基因。密切接触者分离出2株W群脑膜炎奈瑟菌,其PFGE分子分型同源性为100.00%,抗原分子式为W:P1.5-3,10-2:F1-68:ST-17462(CC4821);耐药基因分析显示该菌株喹诺酮类耐药相关基因T91I和β-内酰胺耐药基因PBP2发生突变,对青霉素[最小抑菌浓度(MIC)1.00μg/mL]、左氧氟沙星(MIC 0.12μg/mL)、复方新诺明(MIC 1/19μg/mL)3种抗生素耐药。结论病例与密切接触者分离株扩增出的管家基因一致,该流脑病例由W群ST-17462(CC4821)脑膜炎奈瑟菌引起。新序列型对人类有致病性。Objective To reveal the etiological and molecular characteristics of a meningococcal disease case in Luzhou Sichuan province.Methods Real-time polymerase chain reaction(PCR)was used to detect the specific deoxyribonucleic acid fragments of Neisseria meningitidis isolated from the patient samples,and nest PCR was performed to amplify the house-keeping genes for sequencing.The strains isolated from close contacts of the meningococcal disease case were identified by latex aggregation,pulsed field gel electrophoresis,and drug susceptibility test and sequencing analysis were conducted.Results The strain isolated from the patient samples was identified as N.meningitidis serogroup W via real-time PCR,and three house-keeping genes were amplified by nest PCR.Two strains of N.meningitidis serogroup W isolated from the close contacts shared 100.00%homology in PFGE molecular typing.The typical strain designation was W:P1.5-3,10-2:F1-68:ST-17462(CC4821).The resistance-associated genes analysis showed that isolates harbored the mutations in quinolones resistance related locus T91I of gyrA and in penicillin-binding protein 2(PBP2)loci of penA-552.The isolates were resistant to penicillin[minimal inhibitory concentratio(MIC)1.00μg/mL],levofloxacin(MIC 0.12μg/mL)and sulfamethoxazole(MIC 1/19μg/mL).Conclusion The house-keeping genes of strain from patient samples were coincident with the isolated strains in this study.The meningococcal disease case was caused by N.meningitidis serogroup W:ST-17462(CC4821).The N.meningitidis with new sequence type was pathogenic to humans.

关 键 词：脑膜炎奈瑟菌 W血清型 耐药性 多位点序列分型 ST-17462 

分 类 号：R211[医药卫生—中医学] R378.1