广东韶关烟区烟草镰刀菌根腐病病原鉴定及致病性分析  

Identification and Pathogenicity Analysis of Tobacco Fusarium Root Rot Pathogens in Shaoguan,Guangdong Province

作　　者：沈会芳[1] 邓海滨 杨祁云[1] 张景欣[1] 曾涛 蒲小明[1] 孙大元[1] 林壁润[1] SHEN Huifang;DENG Haibin;YANG Qiyun;ZHANG Jingxin;ZENG Tao;PU Xiaoming;SUN Dayuan;LIN Birun(Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Guangdong Provincial Key Laboratory of New Technique for Plant protection,Guangzhou 510640,China;Guangdong Research Institute of Tobacco Sciences,Shaoguan,Guangdong 512026,China)

机构地区：[1]广东省农业科学院植物保护研究所,广东省植物保护新技术重点实验室,广州510640 [2]广东省烟草科学研究所,广东韶关512026

出　　处：《中国烟草科学》2023年第4期49-57,共9页Chinese Tobacco Science

基　　金：广东省现代农业产业技术体系创新团队项目(2023KJ112);广东省烟草科技计划项目(202201、201908)。

摘　　要：为明确广东韶关烟草镰刀菌根腐病菌种类及致病力,在珠玑、始兴、南雄和乌茎采集根腐病样本,用组织分离法分离病原菌,盆栽接种带菌麦粒测定其致病性,观察菌株形态,基于EF-1α和β-Tub基因序列构建发育树鉴定病原菌。结果表明,获得45株镰刀菌,通过形态特征和分子技术鉴定为茄病镰刀菌Fusarium solani、共享镰刀菌F.commune、藤仓镰刀菌F.fujikuroi和尖孢镰刀菌F.oxysporum,分别占比42.22%,26.67%、20.00%和11.11%,对烟草均有致病性,强弱排列为F.commune≈F.fujikuroi>F.oxysporum>F.solani。本研究可为广东韶关地区烟草镰刀菌根腐病的防治提供依据。To clarity the pathogens of tobacco Fusarium root rot and their pathogenicity,samples showing root rot symptoms from Zhuji,Shixing,Nanxiong and Wujing in Guangdong were collected.Strains were isolated by tissue separation and their pathogenicity to tobacco seedlings was determined by inoculation of wheat grains with fungi in potted tobacco plants.The strains were identified based on morphological characteristics,combined with the phylogenetic analysis method using partial sequences of EF-1αandβ-Tub.45 Fusarium strains were isolated.The results of morphological and molecular biological identification showed that 45 strains belonged to different species,i.e.,Fusarium solani,F.commune,F.fujikuroi and F.oxysporum,and they accounted for 42.22%,26.67%,20.00%and 11.11%,respectively.They all had pathogenicity to tobacco,and the order of pathogenicity was F.commune≈F.fujikuroi>F.oxysporum>F.Solani.Identification of pathogens of tobacco Fusarium root rot may help in establishing the appropriate measures to control this disease.

关 键 词：烟草 镰刀菌根腐病 鉴定 致病性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]