NtJAZ1基因敲除对烤烟Y2001烟碱合成和腺毛发育的影响  

Effects of NtJAZ1 knockout on nicotine synthesis and glandular trichomedevelopment of flue-cured tobacco Y2001

作　　者：侯子航 罗锐 朱文奇 季盈彤 时兴铭 闫筱筱 崔红[1] HOU Zihang;LUO Rui;ZHU Wenqi;JI Yingtong;SHI Xingming;YAN Xiaoxiao;CUI Hong(College of Tobacco,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学烟草学院,郑州市450046

出　　处：《烟草科技》2023年第9期1-9,共9页Tobacco Science & Technology

基　　金：湖南中烟工业有限责任公司合作项目“多腺毛、高烟碱K326种质创制和育种应用研究”(202143000834089)。

摘　　要：烟碱合成和腺毛发育与烟草品质和抗性密切相关,二者受茉莉酸信号的调控。JAZ1是茉莉酸信号途径中关键的负调控基因,为明确NtJAZ1基因敲除对烟碱生物合成、腺毛发育和分泌物积累的影响,采用CRISPR/Cas9技术对烤烟Y2001中的NtJAZ1进行编辑,获得了纯合突变株系HN。与Y2001相比,HN烟苗生长发育无明显差异,叶面腺毛密度降低,但腺头发育完善且比Y2001饱满,腺毛分泌物组分和含量(质量分数)无显著差异。烟苗叶片的烟碱含量提高19.38%,根系烟碱含量提高30.85%,根系中烟碱合成相关基因NtMYC2a、NtMYC2b、NtPMT和NtQPT的表达量极显著增加,烟苗对蚜虫抗性显著增强。NtJAZ1基因敲除虽然对烟腺毛发生有一定影响,但对腺头发育和分泌物合成有明显促进作用,可在不影响叶面分泌物组分和含量的基础上提高烟碱含量和对蚜虫的抗性。因此,NtJAZ1基因是改良烟草品质和抗性的重要靶点之一。Nicotine synthesis and glandular trichome development are closely related to tobacco quality and resistance,both of which are regulated by jasmonic acid signaling.JAZ1 is a key negative regulator gene in the jasmonic acid signaling pathway.To clarify the effects of NtJAZ1 knockout on nicotine biosynthesis,glandular trichome development and secretion accumulation,the CRISPR/Cas9 technology was used to edit NtJAZ1 in flue-cured tobacco Y2001 and a pure mutant plant HN was obtained.Compared to Y2001,HN tobacco seedlings showed no significant differences in growth and development,with a lower density of glandular trichomes on the leaf surface,but the glandular heads were well developed and fuller than the Y2001,and there were no significant differences in the fractions and contents(mass fraction)of glandular trichome secretions.The nicotine content of tobacco seedlings increased by 19.38%in leaves and 30.85%in roots,and the expression levels of NtMYC2a,NtMYC2b,NtPMT and NtQPT,genes related to nicotine synthesis in roots,increased extremely significantly;aphid resistance of tobacco seedlings was significantly improved.Knockout of the NtJAZ1 gene affected tobacco glandular trichome development,but significantly promoted glandular head development and secretion synthesis,which improved nicotine content and aphid resistance without affecting the leaf surface secretion composition and content.Therefore,the NtJAZ1 gene is an important target for tobacco quality and resistance improvement.

关 键 词：烟草 NtJAZ1 基因编辑 烟碱 腺毛 蚜虫抗性 

分 类 号：S572.01[农业科学—烟草工业]