海洋双吲哚化合物FGFC1对心血管血栓模型大鼠血浆FDP、D-D、Plg的影响  

Effects of marine bisindole compound FGFC1 on plasma FDP, D-D and Plg in rats with cardiovascular thrombosis model

作　　者：张添 张惠姝 张林铃 高春丽 张海星 朱玉平 吴文惠[1] ZHANG Tian;ZHANG Huishu;ZHANG Linling;GAO Chunli;ZHANG Haixing;ZHU Yuping;WU Wenhui(Marine Medicine and Health Food Research Center,Shanghai Ocean University,Shanghai 201306,China;Basic Medicine Experimental Teaching Center,Naval Medical University,Shanghai 200433,China)

机构地区：[1]上海海洋大学海洋药物与健康食品研究中心,上海201306 [2]海军军医大学基础医学实验教学中心,上海200433

出　　处：《上海海洋大学学报》2023年第4期874-882,共9页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(82173731);上海市自然科学基金(21ZR1427300)。

摘　　要：建立大鼠心血管血栓模型及观察海洋双吲哚生物碱FGFC1溶解大鼠心血管血栓的纤溶特性。采用异硫氰酸荧光素(Fluorescein 5-isothiocyanate, FITC)标记的纤维蛋白诱导法建立大鼠心血管血栓模型。低场核磁、冰冻切片荧光扫描和苏木精-伊红染色(Hematoxylin-eosin staining, H.E)成像观察大鼠心血管血栓模型建立情况。荧光分析方法检测空白组、对照组、u-PA阳性对照组、FGFC1低、中、高剂量给药组大鼠的血浆荧光强度,ELISA法测定各组大鼠血浆纤维蛋白降解产物(Fibrin degradation product, FDP)含量、D-二聚体(D-Dimer, D-D)含量以及纤溶酶原(Plasminogen, Plg)活性。FITC标记的纤维蛋白原(Fibrinogen, FIB)在还原性SDS-PAGE显示为FITC-α、FITC-β、FITC-γ 3条肽链,FITC-fibrin荧光显微镜下显示绿色荧光,表明标记成功。低场核磁成像显示造模组大鼠心脏组织密度升高,冰冻切片荧光扫描显示心脏血管存在荧光物质斑块,表明心血管血栓模型建成,同时H.E染色组织切片显示造模组心肌纤维异常。FGFC1的2.5、5.0以及10.0 mg/kg剂量给药组大鼠血浆荧光值、Plg活性水平显著低于空白组,FDP水平、D-D水平显著高于空白组。研究表明,海洋双吲哚生物碱FGFC1针对FITC-fibrin诱导形成的大鼠心血管血栓模型能降低其体内Plg活性,促进血栓纤维蛋白降解。To establish a rat cardiovascular thrombosis model and to observe the fibrinolytic properties of the marine bisindole alkaloid FGFC1(Fungi Fibrinolytic Compound 1)in dissolving rat cardiovascular thrombus,a rat cardiovascular thrombosis model was established using fluorescein 5-isothiocyanate(FITC)-labeled fibrin induction method.Myocardial tissue morphology was imaged by low-field NMR,fluorescence scanning of frozen sections and hematoxylin-eosin staining(HE)of paraffin sections.Plasma fluorescence intensity was measured in the blank group,control group,u-PA positive control group and FGFC1 low,medium and high dose groups.,and plasma fibrin degradation product(FDP),D-dimer(D-D)and plasminogen(Plg)were measured by ELISA.FITC-FIB was successfully labeled as FITC-α,FITC-β,FITC-γpeptide chains by reduced SDS-PAGE,and the FITC-fibrin showed green fluorescence under fluorescence microscopy.Low-field NMR imaging showed increased density of cardiac tissue in the modeling group,and fluorescence scanning of frozen sections showed the presence of fluorescent material plaques in cardiac vessels,indicating the establishment of a cardiovascular thrombosis model,while HE staining of tissue sections showed abnormal myocardial fibers in the modeling group.activity levels were significantly lower than those of the blank group,and FDP levels and D-D levels were significantly higher than those of the blank group.FITC-fibrin induced the formation of cardiovascular thrombosis model in rats,and the marine bisindole alkaloid FGFC1 could reduce the Plg activity and promote the degradation of thrombus fibrin in rats.

关 键 词：FGFC1 心血管血栓 组织切片 血浆荧光值 纤溶作用 

分 类 号：R965[医药卫生—药理学]