猪繁殖与呼吸综合征并发副猪嗜血杆菌与猪链球菌感染的鉴别诊断和防治  

Differential Diagnosis and Control of PRRS Complicated by Haemophilus parasuis and Streptococcus suis Infection

作　　者：高艺 郭彦辉 余蕊 韩爽 郝光恩 谢永兴 张潇月 杨玉艾 孙永科 张恒 Gao Yil;Guo Yanhui;Yu Rui;Han Shuang;Hao Guang'en;Xie Yongxing;Zhang Xiaoyue;Yang Yuai;Sun Yongke;Zhang Heng(College of Veterinary Medicine,Yunnan Agricultural University,Kunming,Yunnan 650210,China;Shandong Sinder Technology Co.,Ltd.,Qingdao,Shandong 266104,China;College ofVeterinary Medicine,North West Agriculture and Forestry University,Yangling,Shanxi 712100,China)

机构地区：[1]云南农业大学动物医学院,云南昆明650210 [2]山东信得科技股份有限公司,山东青岛266104 [3]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国动物检疫》2023年第9期25-35,共11页China Animal Health Inspection

基　　金：山东省重点研发计划(重大科技创新工程)项目(2022CXGC020711)。

摘　　要：为确定某规模化猪场引发呼吸道症状的病原,继而制定科学的防治方案,对采集的血清和肺脏样品进行常见呼吸道病原的抗体和抗原检测以及细菌的分离和分型鉴定,并对分离菌株进行药敏试验。病毒抗体检测结果显示,猪繁殖与呼吸综合征病毒(PRRSV)抗体阳性率为60.0%(45/75),猪圆环病毒2型(PCV2)抗体阳性率为98.67%(74/75),猪瘟(CSFV)抗体阳性率为97.33%(73/75)。病毒核酸检测结果显示,PRRSV阳性率为36.0%(9/25),PCV2和CSFV均为阴性。细菌分离鉴定结果显示,培养的细菌符合副猪嗜血杆菌(HPS)和猪链球菌(SS)的形态特征。病原分型鉴定结果显示:感染病原为高致病性PRRSV(HP-PRRSV)和NADC30-like PRRSV;HPS为血清12型,SS为血清9型。药敏试验结果显示,HPS对多西环素、头孢曲松、头孢噻呋较为敏感,SS对青霉素、头孢曲松、头孢噻呋、头孢氨苄较为敏感。结果表明,该猪场疫情由HPPRRSV和NADC30-like PRRSV毒株及HPS-12型和SS-9型混合感染所致。因该猪场免疫的PRRSV CH-1R经典毒株疫苗与分离到的流行毒株不匹配,且免疫后抗体离散度较大,导致疫苗保护力低,继而引起机体免疫抑制并发HPS和SS感染,导致猪大量发病和死亡。通过加强生物安全,进行PRRSV活苗和灭活苗综合紧急免疫,采用敏感药物头孢噻呋进行治疗,病情得到有效控制。本研究为规模化猪场发生PRRSV与其他病原混合感染的鉴别诊断及精准防控提供了成功经验。In order to identify the pathogens causing respiratory symptoms in a large-scale swine farm,and to develop a scientific prevention and control plan,serum and lung samples were collected to detect antibodies and antigens of common respiratory pathogens,meanwhile,bacteria isolation,identification and typing were carried out,then drug susceptibility test was conducted for the isolates.Antibody detection results showed that the positive rates of antibodies against porcine reproductive and respiratory syndrome virus(PRRSV),porcine circovirus type 2(PCV2)and classical swine fever virus(CSFV)were 60.0%(45/75),98.67%(74/75)and 97.33%(73/75),respectively.The results of virus nucleic acid test showed that the positive rate of PRRSV was 36.0%(9/25),both PCV2 and CSFV were detected negative.The results of bacterial isolation and identification showed that the morphological characteristics of the cultured bacteria conformed to those of Haemophilus parasuis(HPS)and Streptococcus suis(SS).The results of bacteria typing showed that highly pathogenic PRRSV(HP-PRRSSV)and NADC30-like PRRSV were contributive to the infection;HPS and SS were determined as serotypes 12 and 9,respectively.The drug sensitivity test results showed that HPS was more sensitive to doxycycline,ceftriaxone and cefotaxime,while SS was more sensitive to penicillin,ceftriaxone,cefotaxime and cephalexin.In conclusion,the outbreak in the farm was caused by coinfection of HP-PRRSV,NADC30-like PRRSV,HPS-12 and SS-9.As the classical vaccine strain of PRRSV CH-1R immunized in the farm failed to match with the isolate,and the dispersion of antibodies after immunization was large,resulting in low protection effect of pigs,then immune suppression complicated by HPS and SS infection,followed by a large number of infected or dead pigs.The outbreak was effectively controlled through strengthening biosecurity,implementing comprehensive emergency vaccination with PRRSV live and inactivated vaccines,and administration of sensitive drug,ceftiofur.A successful experience was pro

关 键 词：猪繁殖与呼吸综合征病毒 副猪嗜血杆菌 猪链球菌 并发感染 鉴别诊断 

分 类 号：S858.288[农业科学—临床兽医学]