Electroacupuncture alleviates orofacial allodynia and anxiety-like behaviors by regulating synaptic plasticity of the CA1 hippocampal region in a mouse model of trigeminal neuralgia  

作　　者：JIA Yuzhi LI Haitao ZHANG Guangming WU Hongyun ZHANG Sishuo ZHI Hongwei WANG Yahan ZHU Jingwen WANG Yifan XU Xiangqing TIAN Caijun CUI Wenqiang 

机构地区：[1]First College of Clinical Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250000,Shandong,China [2]Department of Neurology,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250000,Shandong,China

出　　处：《中国药理学与毒理学杂志》2023年第S01期69-70,共2页Chinese Journal of Pharmacology and Toxicology

基　　金：the National Natural Science Foundation of China(82001190);Natural Sci⁃ence Foundation of Shandong Province(ZR2021LZY016);Natural Science Foundation of Shandong Province(ZR2020MH348);Science and Technology Foundation of Shandong Traditional Chinese Medicine(2020Q035)。

摘　　要：OBJECTIVE To investigate whether electroacupuncture(EA)ameliorates abnormal trigeminal neuralgia(TN)orofacial pain and anxiety-like behavior by altering synaptic plasticity in the hippocampus CA1.METHODS A mouse infraorbital nerve transection model(pTION)of neuropathic pain was established,and EA or sham EA was used to treat ipsilateral acu⁃puncture points(GV20-Baihui and ST7-Xia⁃guan).Golgi-Cox staining and transmission elec⁃tron microscopy(TEM)were administrated to observe the changes of synaptic plasticity in the hippocampus CA1.RESULTS Stable and persistent orofacial allodynia and anxiety-like behav⁃iors induced by pT-ION were related to changes in hippocampal synaptic plasticity.Golgi stain⁃ings showed a decrease in the density of dendritic spines,especially mushroom-type dendritic spines,in hippocampal CA1 neurons of pT-ION mice.TEM results showed that the density of synapses,membrane thickness of the postsynaptic density,and length of the synaptic active zone were decreased,whereas the width of the synaptic cleft was increased in pTION mice.EA attenu⁃ated pT-ION-induced orofacial allodynia and anx⁃iety-like behaviors and effectively reversed the abnormal changes in dendritic spines and syn⁃apse of the hippocampal CA1 region.CONCLU⁃SION EA modulates synaptic plasticity of hippo⁃campal CA1 neurons,and reduces abnormal oro⁃facial pain and anxiety-like behavior,providing evidence for a TN treatment strategy.

关 键 词：trigeminaing neuralgia anxiety ELECTROACUPUNCTURE synaptic plasticity hippocampal CA1 region 

分 类 号：R245.97[医药卫生—针灸推拿学]