藏药德孜阳新醇提物对胃癌细胞SGC-7901迁移的影响  

Effect of the Alcoholic Extract of the Tibetan Medicine Dezhiyangxin on the Migration of SGC-7901,a Gastric Cancer Cell Line

作　　者：易云 江亚惠 杨生玺 拉青才让 赵延礼 Yi Yun;Jiang Yahui;Yang Shengxi;Laqing Cairang;Zhao Yanli(Qinghai University,Xining 810001;Tibetan Hospital of Hainan Tibetan Autonomous Prefecture,Gonghe 813000)

机构地区：[1]青海大学医学部,西宁810001 [2]青海省海南藏族自治州藏医院,共和813000

出　　处：《中国高原医学与生物学杂志》2023年第3期145-157,共13页Journal of Chinese High Altitude Medicine & Biology

基　　金：青海省科技厅项目(No.2022-ZJ-143,No.2022-SF-139)。

摘　　要：目的通过体外细胞实验探讨藏药德孜阳新(DZYX)醇提物对胃癌细胞SGC-7901迁移的影响,并探讨其可能的作用机制,为藏药DZYX的临床应用提供理论依据。方法采用CCK-8法检测药物作用后的细胞活性;采用RTCA实验、Wound healing实验、Transwell实验探讨DZYX醇提物对胃癌细胞SGC-7901迁移的影响;利用共聚焦显微镜观察被DZYX醇提物作用后的细胞骨架变化;用Western Blot法检测被DZYX醇提物作用胃癌细胞后其信号通路蛋白p-AKT、p-mTOR及迁移相关蛋白MMP-2表达水平。基于转录组学进一步验证DZYX醇提物对胃癌细胞迁移的影响,并初步探究DZYX抗胃癌作用的可能机制。结果CCK-8结果显示,DZYX醇提物呈浓度依赖性抑制SGC-7901细胞的增殖(P<0.05);RTCA实验结果表明,不同浓度DZYX醇提物均能抑制SGC-7901迁移,并呈浓度依赖性(P<0.05);Wound healing实验结果显示,DZYX醇提物组伤口愈合率下降;Transwell实验结果显示,药物组迁移细胞数量明显减少(P<0.05);细胞骨架染色结果显示,DZYX醇提物能够影响SGC-7901细胞中肌动蛋白的聚合,抑制微丝形成,重塑细胞骨架;Western Blot结果显示,DZYX醇提物处理胃癌细胞SGC-7901后,PI3K/AKT信号通路中的关键蛋白p-AKT、p-mTOR的表达水平均下降(P<0.05),DZYX醇提物可抑制胃癌细胞SGC-7901中的MMP-2蛋白表达。转录组学结果进一步验证了DZYX醇提物对胃癌细胞迁移的影响,GO功能注释及KEGG通路富集结果显示,DZYX醇提物抗肿瘤机制主要与PI3K/AKT信号通路有关。结论DZYX醇提物可抑制胃癌细胞SGC-7901迁移,作用机制与PI3K/AKT信号通路有关。Objective To investigate the inhibitory effects of Deziyangxin(DZYX)alcoholic extract on the migration of gastric cancer cell in vitro detected by cellular assays and transcriptomics,and to investigate the possible mechanism of DZYX in order to provide theoretical basis for the clinical application of the Tibetan medicine.Methods The CCK-8 assay was used to detect the cellular activity of the medicine.The effect of DZYX alcohol extract on the migration of gastric cancer cells SCC-7901 was investigated using the RTCA cell migration assay,Wound healing assay,and the Transwell assay.Confocal microscopy was used to examine the cytoskeleton changes induced by the DZYX alcohol extract.The signal pathway proteins p-AKT,p-mTOR,and the migration-related protein MMP-2 were detected by Western Blot in gastric cancer cells after the effect of DZYX alcohol extract.The effect of DZYX alcohol extract on migration of gastric cancer cells was further verified based on transcriptomics.Results The CCK-8 showed that DZYX alcohol extract inhibited the proliferation of SGC-7901 cells in a concentration-dependent manner(P<0.05).The results of RTCA showed that the experimental concentrations of DZYX alcohol extracts could inhibit SGC-7901 migration in a concentration-dependent manner(P<0.05).The Wound healing assay showed that the wound healing rate decreased in the DZYX alcohol extract group.The Transwell assay showed a significant decrease in the number of migrating cells in the drug group,and DZYX alcohol extract significantly inhibited the migrating ability of SCC-7901 cells(P<0.05).Cytoskeleton staining showed that DZYX alcohol extract could affect the polymerization of actin in SGC-7901 cells,inhibit microfilament formation,and remodel the cytoskeleton.Western Blot results displayed that the expression levels of p-AKT and p-mTOR,key proteins of the PI3K/AKT signaling pathway,decreased after treatment of SCC-7901 cells by DZYX alcohol extract(P<0.05).Meanwhile,DZYX alcohol extract inhibited the expression of matrix metalloproteinas

关 键 词：乙醇提取物 胃癌 细胞 迁移 

分 类 号：R96[医药卫生—药理学]