金核霉素生物合成途径的体内研究和关键代谢物鉴定  

In vivo Studies on the Biosynthetic Pathway of Aureonuclemycin and Identification of Key Metabolites

作　　者：王飞 金文兵 侯现锋 唐功利 潘海学[1,2] Wang Fei;Jin Wenbing;Hou Xianfeng;Tang Gongli;Pan Haixue(School of Chemistry and Materials Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou 310024;Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032)

机构地区：[1]国科大杭州高等研究院化学与材料科学学院,杭州310024 [2]中国科学院上海有机化学研究所,上海200032

出　　处：《有机化学》2023年第7期2561-2566,共6页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.32070065)资助项目.

摘　　要：在前期获得金核霉素生物合成基因簇的基础上,通过同框删除方法构建了全部基因敲除突变株,确定了在金核霉素生物合成中发挥作用的关键基因.通过突变株和野生型菌株发酵,得到了两个关键的金核霉素类化合物.根据体内基因敲除实验、突变株代谢物分析、关键化合物鉴定结果,提出了金核霉素可能的生物合成途径.On the basis of aureonuclemycin biosynthetic gene cluster obtained in the previous work,in-frame markerless deletion mutants of all genes were constructed,and the key genes that play a role in the biosynthesis of aureonuclemycin were identified.Two key aureonuclemycin-like compounds were successfully obtained from mutant and wild-type strains.According to the results of in vivo experiments,metabolite analysis and identification of key compounds,the possible biosynthetic pathway of aureonuclemycin was proposed.

关 键 词：核苷类抗生素 生物合成 金核霉素 杀草菌素 

分 类 号：TQ450.1[化学工程—农药化工]