藜麦RBOH基因家族的鉴定及表达模式分析  

Genome-Wide Identification and Expression Pattern Analysis of RBOH Gene Family in Quinoa(Chenopodium quinoa Willd.)

作　　者：韩建玮 杨冬冬 曹萌 张侠[1] 郭善利[1,2] 尹海波 HAN Jianwei;YANG Dongdong;CAO Meng;ZHANG Xia;GUO Shanli;YIN Haibo(School of Life Sciences,Yantai University,Yantai 264005,China;College of Grassland Sciences,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005 [2]青岛农业大学草业学院,山东青岛266109

出　　处：《烟台大学学报（自然科学与工程版）》2023年第4期410-418,共9页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省“渤海粮仓”科技示范工程升级版计划项目(2019BHLC001)。

摘　　要：为了探索藜麦RBOH基因(CqRBOH)的结构特征,分析CqRBOH基因功能和表达模式,基于藜麦全基因组数据,通过生物信息学的方法对CqRBOH基因进行鉴定分析。鉴定出9个CqRBOH基因家族成员,根据染色体上位置将其命名为:CqRBOH1-CqRBOH9;亚细胞定位预测结果表明,9个CqRBOH蛋白均定位于质膜上;基于系统进化树将CqRBOH基因家族分为4个亚家族,CqRBOH蛋白序列具有高度保守性,均含有NADPH氧化酶结构域(NADPH-Ox)等4个功能结构域;CqRBOH基因启动子区具有多种与非生物胁迫响应、激素响应以及与生长发育有关的顺式作用元件;转录组数据分析表明CqRBOH3和CqRBOH5在藜麦幼苗中显著表达,部分CqRBOH基因响应干旱、高温和盐胁迫,这些结果为藜麦抗逆机制研究奠定了基础。In order to explore the structural characteristics of CqRBOH and analyze the function and expression pattern of CqRBOH,CqRBOH is identified and analyzed by means of bioinformatics and the whole genome data of quinoa.Nine CqRBOH genes are identified and named as CqRBOH1-CqRBOH9 according to their positions on the chromosome.Subcellular localization prediction shows that all 9 CqRBOH proteins are localized on the plasma membrane.According to the phylogenetic tree,the CqRBOH gene family is divided into four subfamilies,the RBOH protein sequence is highly conservative,all of which contain four functional domains such as NADPH oxidase domain(NADPH-ox).The promoter region of CqRBOH has a variety of cis-acting elements related to abiotic stress response,hormone response and growth.Transcriptome data analysis shows that CqRBOH3 and CqRBOH5 are significantly expressed in quinoa seedlings,and some CqRBOH genes respond to drought,high temperature and salt stress.These results lay a foundation for the study of resistance mechanism of quinoa.

关 键 词：藜麦 呼吸爆发氧化酶同源物 RBOH基因家族 

分 类 号：Q943.2[生物学—植物学] S519[农业科学—作物学]