Langendorff心脏灌流技术(针对心肌缺血再灌注)的应用和发展  

Langendorff cardiac perfusion technique for myocardial ischemia reperfusion applications and developments

作　　者：李楠[1] 张晨[2] 孙艳君 高晟玮 李多静 王心蕊 王保和[3] LI Nan;ZHANG Chen;SUN Yanjun;GAO Shengwei;LI Duojing;WANG Xinrui;WANG Baohe(Tianjin University of Traditional Chinese Medicine,Tianjin 301600,China.;the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300143.;the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300380)

机构地区：[1]天津中医药大学,天津301600 [2]天津中医药大学第二附属医院,天津300143 [3]天津中医药大学第一附属医院,天津300380

出　　处：《中国实验动物学报》2023年第8期1070-1077,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：天津市教委科研计划项目(自然科学)(2021KJ163)。

摘　　要：Langendorff离体心脏灌流模型技术具有可重复性强、给药方便和技术要求低等特点,常被用于研究心肌缺血再灌注损伤。该模型排除了其他器官系统、神经系统或体液等影响。本文对该技术的步骤、动物的选择、灌流模式、灌注液的选择、检测的指标、缺血方式以及缺血和灌流时间的选择等方面进行了综述和优缺点的评价。The Langendorff ex vivo heart perfusion model is a widely used technique used for investigating myocardial ischemia-reperfusion injury(MIRI)because of its notable advantages of high reproducibility,ease of drug administration,and low technical requirements.This model excludes confounding influences from other organ systems,neural pathways,and circulating factors,making it an ideal choice to study MIRI-related diseases.In this review,we provide a comprehensive assessment of the Langendorff perfusion technique,encompassing its procedural steps,animal selection criteria,perfusion modes,choice of perfusion buffer,relevant assessment indices,Methods of inducing ischemia,and considerations for ischemia and reperfusion time intervals.We discuss the strengths and limitations of this model to aid researchers in its proper application in studying MIRI.By analyzing the relevant literature,this review serves as a valuable resource to enhance understanding and implementation of the Langendorff perfusion model in cardiac research.

关 键 词：离体心脏 LANGENDORFF 缺血 再灌注 灌流 

分 类 号：Q95-33[生物学—动物学]