利用海岛棉染色体片段导入系定位纤维品质性状QTL  

QTL Mapping for Fiber Quality Traits Using Gossypium barbadense Chromosome Segment Introgression Lines

作　　者：李兴河 王海涛 刘存敬[1] 唐丽媛[1] 张素君[1] 蔡肖[1] 张香云[1] 张建宏[1] Li Xinghe;Wang Haitao;Liu Cunjing;Tang Liyuan;Zhang Sujun;Cai Xiao;Zhang Xiangyun;Zhang Jianhong(Institute of Cotton,Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area,Ministry of Agriculture and Rural Affairs/Hebei Branch of National Cotton Improvement Center,Shijiazhuang 050051,Hebei,China)

机构地区：[1]河北省农林科学院棉花研究所/农业农村部黄淮海半干旱区棉花生物学与遗传育种重点实验室/国家棉花改良中心河北分中心,河北石家庄050051

出　　处：《作物杂志》2023年第5期1-9,共9页Crops

基　　金：河北省农林科学院基本科研业务费项目(2023070106);河北省农林科学院科技创新专项(2022KJCXZX-MHS-1)。

摘　　要：优质棉育种是当前棉花育种的工作重点,海岛棉染色体片段导入系是定位纤维品质性状QTL、定向改良棉花纤维品质的重要材料。以构建的一套以陆地棉冀棉262为背景的海岛棉海7124染色体片段导入系为材料,对上半部平均长度、断裂比强度、马克隆值、整齐度指数和伸长率5个纤维品质性状进行相关性分析及QTL定位。结果表明,上半部平均长度与断裂比强度、整齐度指数,断裂比强度与整齐度指数均呈极显著正相关,断裂比强度与伸长率呈极显著负相关,可能存在QTL的重叠或连锁。利用459对全部锚定在26条染色体上的SSR标记进行QTL定位分析,鉴定得到155个与纤维品质性状相关的QTL,解释表型变异率在6.57%~38.72%,分别位于At亚组的13条染色体和Dt亚组的7条染色体上,每条染色体上平均检测得到7.75个QTL,其中D5染色体上QTL最多,达30个,与上半部平均长度、马克隆值、伸长率相关的各7个,与整齐度指数相关的6个,与断裂比强度相关的3个;在A9染色体上仅检测到1个与伸长率相关的QTL。本研究鉴定得到的QTL位点与已报道的纤维品质位点部分重合或一致,与之相连锁的分子标记将在优质棉品种选育工作中发挥积极作用。Breeding of superior fiber quality cotton is the focus of current cotton breeding.Gossypium barbadense chromosome segment introgression lines(CSILs)are important materials for QTL mapping of fiber quality traits and directional improvement of fiber quality in cotton.In present study,a Hai 7124 introgression lines with Jimian 262 as background were developed,and the correlation analysis and QTLs mapping of five fiber quality traits were performed by CSIL population.The results showed that the significantly positive correlations among the average fiber length of the upper half(FL),the fiber strength(FS)and uniformity index(FU)were observed,and extremely positive correlation between FS and FU.While,a significant negative correlation between FS and FE was detected.It indicated that QTL overlap or linkage between fiber traits might exist.A total of 459 pairs of SSR markers anchored on all 26 chromosomes were used for QTL mapping analysis.Totally,155 QTLs for five fiber quality traits were identified with explained the phenotypic variation rate of each QTL ranged from 6.57%to 38.72%.These QTLs were located on 13 chromosomes of the At subgroup and seven chromosomes of the Dt subgroup.An average of 7.75 QTLs were detected on each chromosome,among which there were 30 QTLs on Chr.D5,seven QTLs related to FL,MIC and FE,six QTLs related to FU,and three QTLs related to FS.Only one QTL related to FE was detected on Chr.A9.The QTLs identified in this study were partially overlapped or consistent with the reported fiber quality loci,and the linked molecular markers will play an active role in the breeding of high quality cotton varieties.

关 键 词：染色体片段 导入系 QTL定位 纤维品质 优质棉 

分 类 号：S562[农业科学—作物学]